LOCUS       sequence-151719-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of plasmid segregation 
            protein, ParM, as scaffold for ADP biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Kunzelmann S, Webb MR
  TITLE     A fluorescent, reagentless biosensor for ADP based on 
            tetramethylrhodamine-labeled ParM.
  JOURNAL   ACS Chem Biol. 2010 Apr 16;5(4):415-25. doi: 10.1021/cb9003173.
  PUBMED    20158267
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2010-04-16- 16"; volume: "5"; issue: "4"; 
            pages: "415-25"
COMMENT     Sequence Label: pHis17_ParM _2
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(61..78)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(88..105)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatcg ttaatgatga tgatgatgat ggaattctta
       61 gtggtggtgg tggtggtgaa gcttttaatg atgatgatga tgatgggatc ctgattgatt
      121 acctatgaga tacataccgt taactaaatc atattgagag ttattggttt tgaaaaaacg
      181 ttcatcacga atctgtgtgt gtttttttac tgcatcggct attaattctg cgccaccgcc
      241 tataaccata acatgagtat aaccagaaaa ttcattgagc gtatttaata cacgttgctc
      301 aagtttacga agtgcttcat tcattgcttc ggtgactatt gatattttgt tctcatcatt
      361 aattcgttgc ttcagatagt tattatcttt tctgtgaatg attatacagt cagcaagata
      421 gctacttcct tttgttctcg caagagaaag ggcatctttt actgcagatg taaccagaga
      481 gacaccaaga gatgagtctc cgtatatttt actgatcccc gataatttcc ccattacctg
      541 agaaatatct aagttggcgc ccccgagatc tataattaat aaagaatcta actcatccag
      601 ttcttgtaga acttcataac ctgccggtat agattcaggc atgactttta catcttttat
      661 tgtgaatgta tccccgccat ttaatgtaat ttttttccgg aagtttgctt tcttacgctc
      721 aatattttcc gtattgggtt ggttatttct gtcgtaatac tctgtcagag gaagtgtgca
      781 aacaatatcc acttcgctta ccggcagacc actggtcagt aaggcgtgat gcactgcaac
      841 gacattaacg tcgctgtatt gccatgcgat attagttgtg actacagcac acgggctgat
      901 tggatcaaat gaatactgtt cgccgttcag tgtgtagtta aagacctttt tatcaccaaa
      961 agagactgcc cactcgcgtg cgaagctgtt cgggctaatg tgctgtttaa ttgttccgtc
     1021 gctttcctgc cactgtagtt tgatgtttgt tgaaccgtca t
//
