LOCUS       sequence-151730-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of single-cysteine variant of single-stranded DNA-binding
            protein protein, EcSSB, as scaffold for ssDNA biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Dillingham MS, Tibbles KL, Hunter JL, Bell JC, Kowalczykowski SC, 
            Webb MR
  TITLE     Fluorescent single-stranded DNA binding protein as a probe for 
            sensitive, real-time assays of helicase activity.
  JOURNAL   Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. 
            Epub 2008 Jul 3.
  PUBMED    18599625
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1529/biophysj.108.133512"; journalName: "Biophys J"; date: 
            "2008-10"; volume: "95"; issue: "7"; pages: "3330-9"
COMMENT     Sequence Label: pET22b_EcSSB_1
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             605..622
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(675..693)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      689..736
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctttaagaag gagatataca tatggccagc agaggcgtaa acaaggttat tctcgttggt
       61 aatctgggtc aggacccgga agtacgctac atgccaaatt gtggcgcagt tgccaacatt
      121 acgctggcta cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa agaacagact
      181 gaatggcacc gcgttgtgct gttcggcaaa ctggcagaag tggcgagcga atatctgcgt
      241 aaaggttctc aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac cgatcaatcc
      301 ggtcaggatc gctacaccac agaagtcgtg gtgaacgttg gcggcaccat gcagatgctg
      361 ggtggtcgtc agggtggtgg cgctccggca ggtggcaata tcggtggtgg tcagccgcag
      421 ggcggttggg gtcagcctca gcagccgcag ggtggcaatc agttcagcgg cggcgcgcag
      481 tctcgcccgc agcagtccgc tccggcagcg ccgtctaacg agccgccgat ggactttgat
      541 gatgacattc cgttctgagg atccgaattc gagctccgtc gacaagcttg cggccgcact
      601 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      661 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      721 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      841 gccagcgccc tagcgcccgc tccttt
//
