LOCUS       sequence-151739-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of single-cysteine variant of single-stranded DNA-binding
            protein protein, EcSSB, as scaffold for ssDNA biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Fili N, Mashanov GI, Toseland CP, Batters C, Wallace MI, Yeeles JT, 
            Dillingham MS, Webb MR, Molloy JE
  TITLE     Visualizing helicases unwinding DNA at the single molecule level.
  JOURNAL   Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173.
            Epub 2010 Mar 28.
  PUBMED    20350930
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkq173"; journalName: "Nucleic Acids Res"; date: 
            "2010-07"; volume: "38"; issue: "13"; pages: "4448-57"
COMMENT     Sequence Label: pET22b_EcSSB_2
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             642..664
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    679..703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(703..722)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(704..722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(806..825)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg agctcgaatt cggatcctca gaacggaatg tcatcatcaa
      121 agtccatcgg cggctcgtta gacggcgctg ccggagcgga ctgctgcggg cgagactgcg
      181 cgccgccgct gaactgattg ccaccctgcg gctgctgagg ctgaccccaa ccgccctgcg
      241 gctgaccacc accgatattg ccacctgccg gagcgccacc accctgacga ccacccagca
      301 tctgcatggt gccgccaacg ttcaccacga cttctgtggt gtagcgatcc tgaccggatt
      361 gatcggtgca tttacgggta cgcagctgac cttcgatata aacctgagaa cctttacgca
      421 gatattcgct cgccacttct gccagtttgc cgaacagcac aacgcggtgc cattcagtct
      481 gttctttcat ctcgccggtc gctttatcac gccaggattc ggaagtagcc agcgtaatgt
      541 tggcaactgc gccaccattt ggcatgtagc gtacttccgg gtcctgaccc agattaccaa
      601 cgagaataac cttgtttacg cctctgctgg ccatatgtat atctccttct taaagttaaa
      661 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
      721 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
      781 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
      841 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      901 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
      961 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcg
//
