LOCUS       sequence-151743-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of Malonyl-coenzyme A 
            synthetase, RpMatB, as scaffold for ATP biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Vancraenenbroeck R, Webb MR
  TITLE     A Fluorescent, Reagentless Biosensor for ATP, Based on 
            Malonyl-Coenzyme A Synthetase.
  JOURNAL   ACS Chem Biol. 2015 Nov 20;10(11):2650-7. doi: 
            10.1021/acschembio.5b00346. Epub 2015 Sep 21.
  PUBMED    26355992
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00346"; journalName: "ACS Chem Biol"; date: 
            "2015-11-20- 20"; volume: "10"; issue: "11"; pages: "2650-7"
COMMENT     Sequence Label: pTEV5_ RpMatB_1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..112
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat atgtcgtact accatcacca
       61 tcaccatcac gattacgata tcccaactag tgaaaacctg tattttcagg gcgctagcat
      121 gaacgccaac ctgttcgccc gcctgttcga taagctcgac gacccccaca agctcgcgat
      181 cgaaaccgcg gccggggaca agatcagcta cgccgagctg gtggcgcggg cgggccgcgt
      241 cgccaacgtg ctggtggcac gcggcctgca ggtcggcgac cgcgttgcgg cgcaaaccga
      301 gaagtcggtg gaagcgctgg tgctgtatct cgccacggtg cgggccggcg gcgtgtatct
      361 gccgctcaac accgcctata cgctgcacga gctcgattac ttcatcaccg atgccgagcc
      421 gaagatcgtg gtggccgatc cgtccaagcg cgacgggatc gcggcgattg ccgccaaggt
      481 cggcgccacg gtggagacgc ttggccccga cggtcggggc tcgctcaccg atgcggcagc
      541 tggagccagc gaggcgttcg ccacgatcga ccgcggcgcc gatgatctgg cggcgatcct
      601 ctacacctca gggacgaccg gccgctccaa gggcgcgatg ctcagccacg acaatttggc
      661 gtcgaactcg ctgacgctgg tcgattactg gcgcttcacg ccggatgacg tgctgatcca
      721 cgcgctgccg atctatcaca cccatggatt gttcgtggcc agcaacgtca cgctgttcgc
      781 gcgcggatcg atgatcttcc tgccgaagtt cgatcccgac aagatcctcg acctgatggc
      841 gcgcgccacc gtgctgatg
//
