LOCUS       sequence-151753-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of Malonyl-coenzyme A 
            synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Vancraenenbroeck R, Kunzelmann S, Webb MR
  TITLE     Development of a range of fluorescent reagentless biosensors for 
            ATP, based on malonyl-coenzyme A synthetase.
  JOURNAL   PLoS One. 2017 Jun 21;12(6):e0179547. doi: 
            10.1371/journal.pone.0179547. eCollection 2017.
  PUBMED    28636641
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-06-21"; volume: "12(6):e0179547. doi"; pages: " 
            10.1371/journal.pone.0179547. eCollection 2017"
COMMENT     Sequence Label: pTEV5_ RpMatB_2
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatata tgtcgtacta ccatcaccat
       61 caccatcacg attacgatat cccaactagt gaaaacctgt attttcaggg cgctagcatg
      121 aacgccaacc tgttcgcccg cctgttcgat aagctcgacg acccccacaa gctcgcgatc
      181 gaaaccgcgg ccggggacaa gatcagctac gccgagctgg tggcgcgggc gggccgcgtc
      241 gccaacgtgc tggtggcacg cggcctgcag gtcggcgacc gcgttgcggc gcaaaccgag
      301 aagtcggtgg aagcgctggt gctgtatctc gccacggtgc gggccggcgg cgtgtatctg
      361 ccgctcaaca ccgcctatac gctgcacgag ctcgattact tcatcaccga tgccgagccg
      421 aagatcgtgg tggccgatcc gtccaagcgc gacgggatcg cggcgattgc cgccaaggtc
      481 ggcgccacgg tggagacgct tggccccgac ggtcggggct cgctcaccga tgcggcagct
      541 ggagccagcg aggcgttcgc cacgatcgac cgcggcgccg atgatctggc ggcgatcctc
      601 tacacctcag ggacgaccgg ccgcgccaag ggcgcgatgc tcagccacga caatttggcg
      661 tcgaactcgc tgacgctggt cgattactgg cgcttcacgc cggatgacgt gctgatccac
      721 gcgctgccga tctatcacac ccatggattg ttcgtggcca gcaacgtcac gctgttcgcg
      781 cgcggatcga tgatcttcct gccgaagttc gatcccgaca agatcctcga cctgatggcg
      841 cgcgccaccg tgctgatggg tgtgccgacg ttctacacgc ggctct
//
