LOCUS       sequence-151760-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 protein with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pLentiCas9-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccgcctttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
       61 tctttttcgc aacgggtttg ccgccagaac acaggaccgg ttctagagcg ctgccaccat
      121 ggacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
      181 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      241 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      301 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      361 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
      421 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
      481 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
      541 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
      601 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
      661 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
      721 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
      781 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
      841 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
      901 tgccaaactg cagctgagca aggacaccta cgacgacgac
//
