LOCUS       sequence-151761-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 
            and 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 U6-SaDMDR7-U6-SaDMDL2
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..262
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        498..746
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
ORIGIN
        1 ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
       61 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
      121 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      181 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      241 cttgtggaaa ggacgaaaca ccgcagtaat gtgtcatacc ttcgtttaag tactctgtgc
      301 tggaaacagc acagaatcta cttaaacaag gcaaaatgcc gtgtttatct cgtcaacttg
      361 ttggcgagat tttttttata agcttcctgt acagaattcg tctgcaaggg cgaattctgc
      421 agcgtccctg tacaaaaaag caggctttaa aggaaccaat tcagtcgact ggatccggta
      481 ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
      541 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
      601 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
      661 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
      721 atatcttgtg gaaaggacga aacaccgata taatagaaat tattcatgtt taagtactct
      781 gtgctggaaa
//
