LOCUS       sequence-151765-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 
            and 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 U6-SaDMDR7-U6-SaDMDL2
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(110..358)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(338..358)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aatctcgcca acaagttgac gagataaaca cggcattttg ccttgtttaa gtagattctg
       61 tgctgtttcc agcacagagt acttaaacat gaataatttc tattatatcg gtgtttcgtc
      121 ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat
      181 gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc
      241 tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct
      301 ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt
      361 cctgcccgac cttggtaccg gatccagtcg actgaattgg ttcctttaaa gcctgctttt
      421 ttgtacaggg acgctgcaga attcgccctt gcagacgaat tctgtacagg aagcttataa
      481 aaaaaatctc gccaacaagt tgacgagata aacacggcat tttgccttgt ttaagtagat
      541 tctgtgctgt ttccagcaca gagtacttaa acgaaggtat gacacattac tgcggtgttt
      601 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc
      661 atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac
      721 tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt
      781 atctctctaa cagccttgta tcgtatatgc aaat
//
