LOCUS       sequence-151768-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 U6-SaAi9L-U6-SaAi9R
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(109..357)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(337..357)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 atctcgccaa caagttgacg agataaacac ggcattttgc cttgtttaag tagattctgt
       61 gctgtttcca gcacagagta cttaaacaac ccttaatata acttcgtcgg tgtttcgtcc
      121 tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg
      181 atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct
      241 acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc
      301 tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcttc
      361 ctgcccgacc ttggtaccgg atccagtcga ctgaattggt tcctttaaag cctgcttttt
      421 tgtacaggga cgctgcagaa ttcgcccttg cagacgaatt ctgtacagga agcttataaa
      481 aaaaatctcg ccaacaagtt gacgagataa acacggcatt ttgccttgtt taagtagatt
      541 ctgtgctgtt tccagcacag agtacttaaa cgaggatctg cgactctaga gcggtgtttc
      601 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
      661 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
      721 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
      781 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatgg
//
