LOCUS       sequence-151770-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 U6-SaAi9L-U6-SaAi9R
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..301
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        537..785
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
ORIGIN
        1 tttaaaggaa ccaattcagt cgactggatc cggtaccaag gtcgggcagg aagagggcct
       61 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
      121 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
      181 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
      241 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
      301 cgctctagag tcgcagatcc tcgtttaagt actctgtgct ggaaacagca cagaatctac
      361 ttaaacaagg caaaatgccg tgtttatctc gtcaacttgt tggcgagatt ttttttataa
      421 gcttcctgta cagaattcgt ctgcaagggc gaattctgca gcgtccctgt acaaaaaagc
      481 aggctttaaa ggaaccaatt cagtcgactg gatccggtac caaggtcggg caggaagagg
      541 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
      601 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
      661 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      721 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      781 acaccgacga agttatatta agggttgttt aagtactctg tgctggaaac agcacagaat
      841 ctacttaaac aa
//
