LOCUS       sequence-151772-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human MAPK8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: MAPK8 gRNA (BRDN0001487083)
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     112..131
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        211..286
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    342..459
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
       61 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      121 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      181 cgaaacaccg agaaactgca accaacagta gttttagagc tagaaatagc aagttaaaat
      241 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg
      301 cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
      361 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      421 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      481 agagatccac tttggcgccg gctcgagggg gcccgggtgc aaagatggat aaagttttaa
      541 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
      601 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
      661 ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      721 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      781 gtcgccgtga acgttctt
//
