LOCUS       sequence-151777-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of Malonyl-coenzyme A 
            synthetase, RpMatB, as scaffold for ATP biosensor - very weak 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Vancraenenbroeck R, Kunzelmann S, Webb MR
  TITLE     Development of a range of fluorescent reagentless biosensors for 
            ATP, based on malonyl-coenzyme A synthetase.
  JOURNAL   PLoS One. 2017 Jun 21;12(6):e0179547. doi: 
            10.1371/journal.pone.0179547. eCollection 2017.
  PUBMED    28636641
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-06-21"; volume: "12(6):e0179547. doi"; pages: " 
            10.1371/journal.pone.0179547. eCollection 2017"
COMMENT     Sequence Label: pTEV5_ RpMatB_3
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             102..122
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat atgtcgtact
       61 accatcacca tcaccatcac gattacgata tcccaactag tgaaaacctg tattttcagg
      121 gcgctagcat gaacgccaac ctgttcgccc gcctgttcga taagctcgac gacccccaca
      181 agctcgcgat cgaaaccgcg gccggggaca agatcagcta cgccgagctg gtggcgcggg
      241 cgggccgcgt cgccaacgtg ctggtggcac gcggcctgca ggtcggcgac cgcgttgcgg
      301 cgcaaaccga gaagtcggtg gaagcgctgg tgctgtatct cgccacggtg cgggccggcg
      361 gcgtgtatct gccgctcaac accgcctata cgctgcacga gctcgattac ttcatcaccg
      421 atgccgagcc gaagatcgtg gtggccgatc cgtccaagcg cgacgggatc gcggcgattg
      481 ccgccaaggt cggcgccacg gtggagacgc ttggccccga cggtcggggc tcgctcaccg
      541 atgcggcagc tggagccagc gaggcgttcg ccacgatcga ccgcggcgcc gatgatctgg
      601 cggcgatcct ctacacctca gggacggccg gccgctccaa gggcgcgatg ctcagccacg
      661 acaatttggc gtcgaactcg ctgacgctgg tcgattactg gcgcttcacg ccggatgacg
      721 tgctgatcca cgcgctgccg atctatcaca cccatggatt gttcgtggcc agcaacgtca
      781 cgctgttcgc gcgcggatcg atgatcttcc tgccgaagtt cgatcccgac aagatcctcg
      841 acctgatggc gcgcgccacc gtgctgatgg gtgtgccgac gttctacacg cggctcttgc
      901 agagcccgcg gctgaccaag gagacgacgg gcca
//
