LOCUS       sequence-151792-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Iliopoulos O, Kibel A, Gray S, Kaelin WG
  TITLE     Tumour suppression by the human von Hippel-Lindau gene product.
  JOURNAL   Nat Med. 1995 Aug . 1(8):822-6.
  PUBMED    7585187
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            1995 Aug . 1(8):822-6."
COMMENT     Sequence Label: HA-VHL-pRc/CMV
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(822..840)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(823..840)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(860..877)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttacc atggcctacc cctacgacgt gcccgactac gcctccctcg gatccgccac
      121 catgccccgg agggcggaga actgggacga ggccgaggta ggcgcggagg aggcaggcgt
      181 cgaagagtac ggccctgaag aagacggcgg ggaggagtcg ggcgccgagg agtccggccc
      241 ggaagagtcc ggcccggagg aactgggcgc cgaggaggag atggaggccg ggcggccgcg
      301 gcccgtgctg cgctcggtga actcgcgcga gccctcccag gtcatcttct gcaatcgcag
      361 tccgcgcgtc gtgctgcccg tatggctcaa cttcgacggc gagccgcagc cctacccaac
      421 gctgccgcct ggcacgggcc gccgcatcca cagctaccga ggtcaccttt ggctcttcag
      481 agatgcaggg acacacgatg ggcttctggt taaccaaact gaattatttg tgccatctct
      541 caatgttgac ggacagccta tttttgccaa tatcacactg ccagtgtata ctctgaaaga
      601 gcgatgcctc caggttgtcc ggagcctagt caagcctgag aattacagga gactggacat
      661 cgtcaggtcg ctctacgaag atctggaaga ccacccaaat gtgcagaaag acctggagcg
      721 gctgacacag gagcgcattg cacatcaacg gatgggagat tgaattctgc agatatccat
      781 cacactggcg gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat
      841 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      901 ccctcccccg tgccttcctt gaccctggaa ggtgccactc c
//
