LOCUS       sequence-151797-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2,
            Klf4 and Myc for iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: TetO-FUW-OSKM
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(77..133)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ctccgccgcc gccccccgaa gcttgctgcg ggcccggcgg cttcagctcc gtctccatca
       61 tgttatacat gcatgcaggc ccggggtttt cttcaacatc tcctgcttgc tttaacagag
      121 agaagttcgt ggcgccgctg ccgctagcgt ttgaatgcat gggagagccc agagcagtga
      181 cgggaacaga gggaaaggcc tcgccctcag gaaaagggac tgagtagagt gtggtgaagt
      241 gggggcttcc atagcctggg gtgccaaagt ggggacctgg gggcagagga aaggatacag
      301 ccccccctgg gaaaggtgtc cctgtagcct catactcttc tcgttgggaa tactcaatac
      361 ttgatctttt gcccttctgg cgccggttac agaaccatac tcgaaccaca tccttctcta
      421 gcccaagctg attggcgatg tgagtgatct gctgtaggga gggcttcggg cacttcagaa
      481 acatggtctc cagactccac ctcacacggt tctcaatgct agttcgcttt ctcttccggg
      541 cctgcaccag ggtctccgat ttgcatatct cctgaaggtt ctcattgttg tcggcttcct
      601 ccacccactt ctccagcagg ggccgcagct tacacatgtt cttaaggctg agctgcaagg
      661 cctcgaagcg acagatggtg gtctggctga acacctttcc aaagagaacg cccagggtga
      721 gccccacgtc ggcctgggtg taccccaagg tgatcctctt ctgcttcagc agcttggcaa
      781 actgttctag ctccttctgc agggctttca tgtcctggga ctcctcggga gttggttcca
      841 ccttctccaa cttcacggca ttggggcggt cggcacaggg ctcagaggag
//
