LOCUS       sequence-151803-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J
  TITLE     Molecular interpretation of ERK signal duration by immediate early 
            gene products.
  JOURNAL   Nat Cell Biol. 2002 Aug . 4(8):556-64.
  PUBMED    12134156
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Aug . 4(8):556-64."
COMMENT     Sequence Label: pcDNA3-FLAG-Fos WT
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcacca
      121 tggactacaa agacgatgac gacaagttct cgggtttcaa cgcggactac gaggcgtcat
      181 cctcccgctg cagtagcgcc tccccggccg gggacagcct ttcctactac cattccccag
      241 ccgactcctt ctccagcatg ggctcccctg tcaacacaca ggacttttgc gcagatctgt
      301 ccgtctctag tgccaacttt atccccacgg tgacagccat ctccaccagc ccagacctgc
      361 agtggctggt gcagcccact ctggtctcct ccgtggcccc atcgcagacc agagcgcccc
      421 atccttacgg actccccacc ccgtcgaccg gggcttacgc cagagcggga gtggtgaaga
      481 ccatgtcagg cggcagagcg cagagcatcg gcagaagggg caaagtagag cagctatctc
      541 ctgaagagga agagaaacgg agaatccgaa gggaaaggaa taagatggct gcagccaagt
      601 gccggaatcg gaggagggag ctgacagata cgctccaagc ggagacagat caacttgaag
      661 acgagaagtc tgcgttgcag accgagattg ccaatctact gaaagagaag gaaaaactgg
      721 agtttatttt ggcagcccac cgacctgcct gcaagatccc caatgacctg ggcttcccag
      781 aggagatgtc tgtgacctcc ctggacttga ctgggggtct gcctgaggct accaccccag
      841 agtctgagga ggccttcacc ctgcctcttc tcaatgaccc tgagcccaag ccatccttgg
      901 agccggtcaa gaacattagc aacatggagc tgaaggctga accctttgat gacttcttgt
      961 ttccggc
//
