LOCUS       sequence-151808-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pU6-1 (GB1204)
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    261..282
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      285..328
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(332..350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(578..597)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tacttcgact ctaaccatat ataaactcag ctgctttctt tacctaagcg ctgtggtact
       61 tttgaagatt gttaatagct taaatgggcc tattttagaa aaaggcctat ccctcgcttc
      121 cttctcaagc ccatttatat tattgaattt tttcccaact aaagatttta ttttcttatg
      181 caagtctcac tcacactcac gtaaatttca aatataatcg tggtggaact aaaacaatga
      241 cctcccgaga ccactcatcg ctctagaagc ggccgcgaat tccagaaatc atccttagcg
      301 aaagctaagg atttttttta tctgaaattc tgcctcgtga tacgcctatt tttataggtt
      361 aatgtcatga taataatggt ttcttagacg tcaggtggca ctcgagttga tcgggcacgt
      421 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      481 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      541 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      601 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      661 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      721 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      781 ttgttacacc gttttccatg agcaaactga aacgttttca tcactctgga gtgaatacca
      841 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
      901 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtatcag ccaatccctg
      961 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1021 tttcaccatg ggc
//
