LOCUS       sequence-151814-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the human codon optimized CDS of Cas9 protein with mutated 
            (D10A, H840A) and inactivated catalytic domains as a level 0 
            GoldenBraid part for C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pdCas9 (GB1079)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..78
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattgggcg atgagtggtc tcgaatgccc
       61 aagaagaaga ggaaggtgga caagaagtac tccattgggc tcgctatcgg cacaaacagc
      121 gtcggctggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg
      181 ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc
      241 ggggaaacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga
      301 aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac
      361 tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc
      421 cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata
      481 tatcatctga ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat
      541 ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac
      601 ccagacaaca gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt
      661 ttcgaagaga acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg
      721 ctgtccaaat cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac
      781 ggcctgtttg gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac
      841 ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc
      901 gacaatctgc tggcccagat c
//
