LOCUS       sequence-151815-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the human codon optimized CDS of Cas9 protein with mutated 
            (D10A, H840A) and inactivated catalytic domains as a level 0 
            GoldenBraid part for C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pdCas9 (GB1079)
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcgttgggag ctctcccata tggtcgacct gcaggcggcc gcgaattcac tagtgattgg
       61 gcgatgagtg gtctcgcgaa tcagccctgc tgtctccacc gagctgagag aggtcgattc
      121 ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg
      181 tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc
      241 ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat
      301 ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc
      361 ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca
      421 cgaacagctg cttctgctca ttatcttcgg gagatccttt gagcttttca tagtggctgg
      481 ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct
      541 cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg
      601 gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga
      661 tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc
      721 ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga
      781 ctgtaggaga atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca
      841 gcttgtcgct gttccttttc gggaggatac tttccttgga gaagcctccg gtctgtactt
      901 cggtcttttt aacgatgttc acctgcggca tggacaggac cttccggact gtcgcgaaat
      961 ccctaccctt gtcccac
//
