LOCUS       sequence-151827-        9019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti6 destination vector encoding C-terminally EGFP-tagged human 
            doublecortin (DCX; GenBank: NM_178152).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ettinger A, van Haren J, Ribeiro SA, Wittmann T
  TITLE     Doublecortin Is Excluded from Growing Microtubule Ends and 
            Recognizes the GDP-Microtubule Lattice.
  JOURNAL   Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 
            10.1016/j.cub.2016.04.020. Epub 2016 May 26.
  PUBMED    27238282
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2016.04.020"; journalName: "Curr Biol"; date: 
            "2016-06-20- 20"; volume: "26"; issue: "12"; pages: "1549-55"
COMMENT     Sequence Label: pLenti6-DCX-EGFP
FEATURES             Location/Qualifiers
     source          1..9019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2274..2294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2439..2463
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3692..3701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3698..3700,3701..3703,3704..4417)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3743..3764)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4004..4023)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4351..4372
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4452..4476)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4529..4570
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4607..4627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4612..4941
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4792..4927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4989..5036
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5055..5453
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5540..5773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5845..5966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5882..5901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5936..5955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6006..6141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6161..6180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6162..6180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6190..6207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6190..6206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6199..6221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6348..6803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6435..6454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6645..6666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6829..6933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6934..7002,7003..7794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7152..7171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7965..8553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8454..8473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8707..8724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8841..8862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8877..8882,8883..8900,8901..8907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8915..8931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8920..8942
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8939..8955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8939..8955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8974..8994
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8976..8994
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaccgtcag
     2341 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctagagg
     2401 atccactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagcag
     2461 gctccgcggc cctagcgcta ccggactcag atctcgagct caagcttcga attaattcgc
     2521 ccttctcggt accatggaac ttgattttgg acactttgac gaaagagata agacatccag
     2581 gaacatgcga ggctcccgga tgaatgggtt gcctagcccc actcacagcg cccactgtag
     2641 cttctaccga accagaacct tgcaggcact gagtaatgag aagaaagcca agaaggtacg
     2701 tttctaccgc aatggggacc gctacttcaa ggggattgtg tacgctgtgt cctctgaccg
     2761 ttttcgcagc tttgacgcct tgctggctga cctgacgcga tctctgtccg acaacatcaa
     2821 cctgcctcag ggagtgcgtt acatttacac cattgatgga tccaggaaga tcggaagcat
     2881 ggatgaactg gaggaagggg aaagctatgt ctgttcctca gacaacttct ttaaaaaggt
     2941 ggagtacacc aagaatgtca atcccaactg gtctgtcaac gtaaaaacat ctgccaatat
     3001 gaaagccccc cagtctttgg ctagcagcaa cagtgcacag gccagggaga acaaggactt
     3061 tgtgcgcccc aagctggtta ccatcatccg cagtggggtg aagcctcgga aggctgtgcg
     3121 tgtgcttctg aacaagaaga cagcccactc ttttgagcaa gtcctcactg atatcacaga
     3181 agccatcaaa ctggagaccg gggttgtcaa aaaactctac actctggatg gaaaacaggt
     3241 aacttgtctc catgatttct ttggtgatga tgatgtgttt attgcctgtg gtcctgaaaa
     3301 atttcgctat gctcaggatg atttttctct ggatgaaaat gaatgccgag tcatgaaggg
     3361 aaacccatca gccacagctg gcccaaaggc atccccaaca cctcagaaga cttcagccaa
     3421 gagccctggt cctatgcgcc gaagcaagtc tccagctgac tcaggtaacg accaagacgc
     3481 aaacggaacc tccagcagcc agctctctac ccccaagtct aagcagtctc ccatctctac
     3541 gcccaccagt cctggcagcc tccggaagca caaggacctg tacctgcctc tgtccttgga
     3601 tgactcggac tcgcttggtg attccatggg taccaagaag ggcgaattaa ttctgcagtc
     3661 gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     3721 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3781 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3841 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3901 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3961 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4021 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4081 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4141 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4201 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4261 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     4321 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4381 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggcctcgagg gtaccaaggg
     4441 tgggcgcgcc gacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     4501 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     4561 cgattctacg cgtaccggtt agtaatgagt ttggaattaa ttctgtggaa tgtgtgtcag
     4621 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4681 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4741 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4801 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4861 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4921 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4981 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     5041 tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     5101 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     5161 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     5221 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     5281 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     5341 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     5401 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacaat
     5461 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5521 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5581 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5761 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5821 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5941 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6001 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6061 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6121 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6181 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6241 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6301 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6781 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6841 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6901 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6961 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7021 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7081 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7141 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7201 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7261 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7321 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7381 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7441 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7501 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7561 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7621 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7681 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7741 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7801 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7861 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8101 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8581 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8641 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8701 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8761 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8821 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8881 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8941 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     9001 aagctggagc tgcaagctt
//
