LOCUS       sequence-151829-        5050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector that expresses the HIV-1 GAG protein 
            with HRAS fused to it.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Eyckerman S, Titeca K, Van Quickelberghe E, Cloots E, Verhee A, 
            Samyn N, De Ceuninck L, Timmerman E, De Sutter D, Lievens S, Van 
            Calenbergh S, Gevaert K, Tavernier J
  TITLE     Trapping mammalian protein complexes in viral particles.
  JOURNAL   Nat Commun. 2016 Apr 28;7:11416. doi: 10.1038/ncomms11416.
  PUBMED    27122307
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-28"; volume: "7:11416. doi"; pages: " 
            10.1038/ncomms11416"
COMMENT     Sequence Label: pMET7-GAG-HRAS
FEATURES             Location/Qualifiers
     source          1..5050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..1531
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     polyA_signal    2176..2310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2213..2232)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2267..2286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2349..2366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2520..3108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2600..2619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3279..4070,4071..4139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3902..3921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4140..4244)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4284..4304)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(4289..4618,4619..4634,4635..4903)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      4469..4604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             4635..4903
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          4933..5026
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
ORIGIN
        1 ctctaaaagc tgcgggcccg ctagcgccac catgggtgcg agagcgtcag tattaagcgg
       61 gggagaatta gatcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      121 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
      181 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
      241 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
      301 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
      361 aagtaagaaa aaagcacagc aagcagcagc tgacacagga cacagcaatc aggtcagcca
      421 aaattaccct atagtgcaga acatccaggg gcaaatggta catcaggcca tatcacctag
      481 aactttaaat gcatgggtaa aagtagtaga agagaaggct ttcagcccag aagtgatacc
      541 catgttttca gcattatcag aaggagccac cccacaagat ttaaacacca tgctaaacac
      601 agtgggggga catcaagcag ccatgcaaat gttaaaagag accatcaatg aggaagctgc
      661 agaatgggat agagtgcatc cagtgcatgc agggcctatt gcaccaggcc agatgagaga
      721 accaagggga agtgacatag caggaactac tagtaccctt caggaacaaa taggatggat
      781 gacacataat ccacctatcc cagtaggaga aatctataaa agatggataa tcctgggatt
      841 aaataaaata gtaagaatgt atagccctac cagcattctg gacataagac aaggaccaaa
      901 ggaacccttt agagactatg tagaccgatt ctataaaact ctaagagccg agcaagcttc
      961 acaagaggta aaaaattgga tgacagaaac cttgttggtc caaaatgcga acccagattg
     1021 taagactatt ttaaaagcat tgggaccagg agcgacacta gaagaaatga tgacagcatg
     1081 tcagggagtg gggggacccg gccataaagc aagagttttg gctgaagcaa tgagccaagt
     1141 aacaaatcca gctaccataa tgatacagaa aggcaatttt aggaaccaaa gaaagactgt
     1201 taagtgtttc aattgtggca aagaagggca catagccaaa aattgcaggg cccctaggaa
     1261 aaagggctgt tggaaatgtg gaaaggaagg acaccaaatg aaagattgta ctgagagaca
     1321 ggctaatttt ttagggaaga tctggccttc ccacaaggga aggccaggga attttcttca
     1381 gagcagacca gagccaacag ccccaccaga agagagcttc aggtttgggg aagagacaac
     1441 aactccctct cagaagcagg agccgataga caaggaactg tatcctttag cttccctcag
     1501 atcactcttt ggcagcgacc cctcgtcaca aggcgggtcg acgagctcag aattcaccga
     1561 atacaagctt gttgttgttg gcgccggcgg tgtgggcaag agtgcgctga ccatccagct
     1621 gatccagaac cattttgtgg acgaatacga ccccactata gaggattcct accggaagca
     1681 ggtggtcatt gatggggaga cgtgcctgtt ggacatcctg gataccgccg gccaggagga
     1741 gtacagcgcc atgcgggacc agtacatgcg caccggggag ggcttcctgt gtgtgtttgc
     1801 catcaacaac accaagtctt ttgaggacat ccaccagtac agggagcaga tcaaacgggt
     1861 gaaggactcg gatgacgtgc ccatggtgct ggtggggaac aagtgtgacc tggctgcacg
     1921 cactgtggaa tctcggcagg ctcaggacct cgcccgaagc tacggcatcc cctacatcga
     1981 gacctcggcc aagacccggc agggagtgga ggatgccttc tacacgttgg tgcgtgagat
     2041 ccggcagcac aagctgcgga agctgaaccc tcctgatgag agtggccccg gctaagcggc
     2101 cgcataatct agagaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2161 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2221 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2281 ctcatcaatg tatcttatca tgtctggatc cccgggtacc gagctcgaat taattcctct
     2341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2701 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2941 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4261 gtgccacctg caagcttggc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4381 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4441 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4501 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4561 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4621 gctcctccga tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     4681 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     4741 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     4801 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     4861 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctc tgaaaaacca
     4921 gaaagttaac tggtaagttt agtctttttg tcttttattt caggtcccgg atccggtggt
     4981 gcaaatcaaa gaactgctcc tcagtggatg ttgcctttac ttctaggcct gtacggaagt
     5041 gttacttctg
//
