LOCUS       sequence-151833-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial vector for cloning and propagation in DH5alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pET11a-CA(A14C, E45C, A92E)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             702..724
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    739..763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(763..782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(764..782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaactcttgc tttatggccg ggtcccccca ctccctgaca tgctgtcatc atttcttcta
       61 gtgtcgctcc tggtcccaat gcttttaaaa tagtcttaca atctgggttc gcattttgga
      121 ccaacaaggt ttctgtcatc caatttttta cctcttgtga agcttgctcg gctcttagag
      181 ttttatagaa tcggtctaca tagtctctaa agggttcctt tggtccttgt cttatgtcca
      241 gaatgctggt agggctatac attcttacta ttttatttaa tcccaggatt atccatcttt
      301 tatagatttc tcctactggg ataggtggat tatgtgtcat ccatcctatt tgttcctgaa
      361 gggtactagt agttcctgct atgtcacttc cccttggttc tctcatctgg cctggttcaa
      421 taggccctgc atgcactgga tgcaatctat cccattctgc agcttcctca ttgatggtct
      481 cttttaacat ttgcatggct gcttgatgtc cccccactgt gtttagcatg gtatttaaat
      541 cttgtggggt ggctccgcat gataatgctg aaaacatggg tattacttct gggctgaaag
      601 ccttctcttc tactactttt acccatgcat ttaaagttct aggtgatatg cactgatgta
      661 ccatttgccc ctgcaggttc tgaacgatcg gcatatgtat atctccttct taaagttaaa
      721 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
      781 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
      841 ggcgccacag
//
