LOCUS       sequence-151836-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculoviral entry vector to produce Bac-to-bac baculoviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pFastBac1-OSF-TRIM5alpha(African green monkey
            ...
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..43
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             80..103
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             149..172
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aagggccacc atggctagct ggagccaccc gcagttcgag aaaggtggag gtgcccgagg
       61 tggatcggga ggtggatcgt ggagccaccc gcagttcgaa aaaggattcg attacaagga
      121 tgacgatgac aagggtacaa gatctccgct ggaagtcctg ttccagggcc ccatggcttc
      181 tggaatcctg gttaatgtaa aggaggaggt gacctgtccc atctgcctgg aactcctgac
      241 agaacccctg agtctgccct gcggccacag cctctgccaa gcgtgcatca ctgcaaacca
      301 caaggagtcc atgctataca aagaagaaga gagaagctgc cctgtgtgcc ggatcagtta
      361 ccagcctgag aatatacagc ctaatcggca tgtggccaac atagtggaga agctcagaga
      421 ggtcaagttg agcccagaag aggggcagaa ggttgatcac tgtgcacgcc atggagagaa
      481 actcctactc ttctgtcagg aggacagcaa ggtcatttgc tggctttgtg agcggtctca
      541 ggagcaccgt ggtcaccaca ctttcctcat ggaggaggtt gcccaggagt accatgtgaa
      601 gctccagaca gctctggaga tgctgaggca gaagcagcag gaagctgaaa agttggaagc
      661 tgacatcaga gaagagaaag cttcctggaa gattcaaata gactacgaca aaaccaacgt
      721 ctcggcagat tttgagcaac tgagagagat cctggactgg gaggagagca atgagctgca
      781 gaacctggag aaggaggaag aagacattct gaa
//
