LOCUS       sequence-151838-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for cloning and propagation in DH5alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pDONR221-OSF-TRIM5-21R(1-300)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             120..143
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             180..203
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             210..233
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             249..272
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
       61 ccaactttgt acaaaaaagc aggcttccga tcgacggtat cgatgccacc atggctagct
      121 ggagccaccc gcagttcgag aaaggtggag gtgcccgagg tggatcggga ggtggatcgt
      181 ggagccaccc gcagttcgaa aaaggattcg attacaagga tgacgatgac aagggtacaa
      241 gatctccgct ggaagtcctg ttccagggcc ctatggcttc agcagcacgc ttgacaatga
      301 tgtgggagga ggtcacatgc cctatctgcc tggacccctt cgtggagcct gtgagcatcg
      361 agtgtggcca cagcttctgc caggaatgca tctctcaggt tgggaaaggt gggggcagcg
      421 tctgtcctgt gtgccggatc agttaccagc ctgagaacat acagcctaat cggcatgtag
      481 ccaacatagt ggagaagctc agggaggtca agttgagccc agaagaggga cagaaggttg
      541 atcactgtgc acgccatgga gagaaactcc tactcttctg tcaggaggac agcaaggtca
      601 tttgctggct ttgtgagcgg tctcaggagc accgtggtca ccacactttc ctcatggagg
      661 aggttgccca ggagtaccat gtgaagctcc agacagctct ggagatgctg aggcagaagc
      721 agcaggaagc tgaaaagttg gaagctgaca tcagagaaga gaaagcttcc tggaagattc
      781 aaatagacta cgacaaaacc aacgtctcgg cagattttga gcaactgaga gagatcctgg
      841 actgggagga gagcaatgag ctgcagaacc tggagaagga ggaagaa
//
