LOCUS       sequence-151840-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for cloning and propagation in DH5alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pDONR221-OSF-TRIM5-21R
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      111..120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             126..149
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             186..209
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             216..239
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             255..278
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc ttccgatcga cggtatcgat gccaccatgg
      121 ctagctggag ccacccgcag ttcgagaaag gtggaggtgc ccgaggtgga tcgggaggtg
      181 gatcgtggag ccacccgcag ttcgaaaaag gattcgatta caaggatgac gatgacaagg
      241 gtacaagatc tccgctggaa gtcctgttcc agggccctat ggcttcagca gcacgcttga
      301 caatgatgtg ggaggaggtc acatgcccta tctgcctgga ccccttcgtg gagcctgtga
      361 gcatcgagtg tggccacagc ttctgccagg aatgcatctc tcaggttggg aaaggtgggg
      421 gcagcgtctg tcctgtgtgc cggatcagtt accagcctga gaacatacag cctaatcggc
      481 atgtagccaa catagtggag aagctcaggg aggtcaagtt gagcccagaa gagggacaga
      541 aggttgatca ctgtgcacgc catggagaga aactcctact cttctgtcag gaggacagca
      601 aggtcatttg ctggctttgt gagcggtctc aggagcaccg tggtcaccac actttcctca
      661 tggaggaggt tgcccaggag taccatgtga agctccagac agctctggag atgctgaggc
      721 agaagcagca ggaagctgaa aagttggaag ctgacatcag agaagagaaa gcttcctgga
      781 agattcaaat agactacgac aaa
//
