LOCUS       sequence-151854-        6095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV5-AML1B is the original plasmid used in Meyers et al 1995. 
            However, please note that the insert sequence has now been 
            identified as AML1C..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Meyers S, Lenny N, Hiebert SW
  TITLE     The t(8;21) fusion protein interferes with AML-1B-dependent 
            transcriptional activation.
  JOURNAL   Mol Cell Biol. 1995 Apr . 15(4):1974-82.
  PUBMED    7891692
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1995 Apr . 15(4):1974-82."
COMMENT     Sequence Label: pCMV5-AML1B
FEATURES             Location/Qualifiers
     source          1..6095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        81..460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        461..664
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     615..635
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2199..2821
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2398..2417)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2845..2865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2850..3179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3030..3165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3217..3236)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3218..3236)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3250..3266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3250..3266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3263..3285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3274..3290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3298..3304,3305..3322,3323..3328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3343..3364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3481..3498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3652..4240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3732..3751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4411..5202,5203..5271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5034..5053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5272..5376)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5403..5858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5490..5509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5700..5721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5984..6006
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5998..6015
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5999..6015
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
       61 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
      121 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      181 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      241 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      301 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
      361 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
      421 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      481 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      541 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
      601 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      661 agctcgttta gtgaaccgtc agaattcgcg atggcttcag acagcatatt tgagtcattt
      721 ccttcgtacc cacagtgctt catgagagaa tgcatacttg gaatgaatcc ttctagagac
      781 gtccacgatg ccagcacgag ccgccgcttc acgccgcctt ccaccgcgct gagcccaggc
      841 aagatgagcg aggcgttgcc gctgggcgcc ccggacgccg gcgctgccct ggccggcaag
      901 ctgaggagcg gcgaccgcag catggtggag gtgctggccg accacccggg cgagctggtg
      961 cgcaccgaca gccccaactt cctctgctcc gtgctgccta cgcactggcg ctgcaacaag
     1021 accctgccca tcgctttcaa ggtggtggcc ctaggggatg ttccagatgg cactctggtc
     1081 actgtgatgg ctggcaatga tgaaaactac tcggctgagc tgagaaatgc taccgcagcc
     1141 atgaagaacc aggttgcaag atttaatgac ctcaggtttg tcggtcgaag tggaagaggg
     1201 aaaagcttca ctctgaccat cactgtcttc acaaacccac cgcaagtcgc cacctaccac
     1261 agagccatca aaatcacagt ggatgggccc cgagaacctc gaagacatcg gcagaaacta
     1321 gatgatcaga ccaagcccgg gagcttgtcc ttttccgagc ggctcagtga actggagcag
     1381 ctgcggcgca cagccatgag ggtcagccca caccacccag cccccacgcc caaccctcgt
     1441 gcctccctga accactccac tgcctttaac cctcagcctc agagtcagat gcaggataca
     1501 aggcagatcc aaccatcccc accgtggtcc tacgatcagt cctaccaata cctgggatcc
     1561 attgcctctc cttctgtgca cccagcaacg cccatttcac ctggacgtgc cagcggcatg
     1621 acaaccctct ctgcagaact ttccagtcga ctctcaacgg cacccgacct gacagcgttc
     1681 agcgacccgc gccagttccc cgcgctgccc tccatctccg acccccgcat gcactatcca
     1741 ggcgccttca cctactcccc gacgccggtc acctcgggca tcggcatcgg catgtcggcc
     1801 atgggctcgg ccacgcgcta ccacacctac ctgccgccgc cctaccccgg ctcgtcgcaa
     1861 gcgcagggag gcccgttcca agccagctcg ccctcctacc acctgtacta cggcgcctcg
     1921 gccggctcct accagttctc catggtgggc ggcgagcgct cgccgccgcg catcctgccg
     1981 ccctgcacca acgcctccac cggctccgcg ctgctcaacc ccagcctccc gaaccagagc
     2041 gacgtggtgg aggccgaggg cagccacagc aactccccca ccaacatggc gccctccgcg
     2101 cgcctggagg aggccgtgtg gcggccctac tgaattcaga tctggtacca cgcgtatcga
     2161 taagcttgca tgcctgcagg tcgactctag aggatcccgg gtggcatccc tgtgacccct
     2221 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     2281 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     2341 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     2401 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     2461 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     2521 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     2581 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     2641 tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa ctataccagc
     2701 aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg ggacatttga
     2761 gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg cctgttgaat
     2821 tgggtacgcg gccagcttgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     2881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2941 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3001 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3061 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3121 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3181 agctcctcga ggaactgaaa aaccagaaag ttaattccct atagtgagtc gtattaaatt
     3241 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3301 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3361 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3421 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3481 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3541 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3601 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3661 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3721 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3781 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3841 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3901 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3961 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4021 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4081 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4141 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4201 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4261 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4321 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4381 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4441 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4501 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4561 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4621 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4681 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4741 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4801 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4861 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4921 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4981 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5041 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5101 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5161 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5221 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5281 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5341 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5401 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5461 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5521 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5581 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5641 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5701 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5761 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5821 taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc
     5881 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5941 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     6001 aaaacgacgg ccagtgccaa gctgatctat acattgaatc aatattggca attagccata
     6061 ttagtcattg gttatatagc ataaatcaat attgg
//
