LOCUS       sequence-151857-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes chloramphenicol resistance gene for 
            positive selection and a toxin gene (relE) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-242
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     206..225
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(292..394)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      599..685
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
       61 cattgccata cgtaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      121 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      181 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      241 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      301 cttagctcct gaaaatctcg acaactcaaa aaatacgccc ggtagtgatc ttatttcatt
      361 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
      421 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
      481 cgtcacaggt aggcgcgccg aagttcctat actccgcggc ccaaagtgcc acctgcatcg
      541 atggcccccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
      601 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      661 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      721 gcccggaggg tggcgg
//
