LOCUS       sequence-151860-        5472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human LIN28A in mammalian cells using retrovirus system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Tanabe K, Nakamura M, Narita M, Takahashi K, Yamanaka S
  TITLE     Maturation, not initiation, is the major roadblock during 
            reprogramming toward pluripotency from human fibroblasts.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12172-9. doi: 
            10.1073/pnas.1310291110. Epub 2013 Jun 28.
  PUBMED    23812749
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1310291110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-23- 23"; volume: "110"; issue: "30"; pages: "12172-9"
COMMENT     Sequence Label: pMXs-hLIN28A
FEATURES             Location/Qualifiers
     source          1..5472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             27..443
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     373..395
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     411..427
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    453..827
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     765..781
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    878..902
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1569..1593)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             1910..2379
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2509..2526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2680..3268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2760..2779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3432..4223,4224..4292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4055..4074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4293..4397)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             4427..5020
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5083..5440
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
       61 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
      121 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
      181 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
      241 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
      301 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
      361 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
      421 ccctcactcc ttctctaggc gcccccatat ggccatatga gatcttatat ggggcacccc
      481 cgccccttgt aaacttccct gaccctgaca tgacaagagt tactaacagc ccctctctcc
      541 aagctcactt acaggctctc tacttagtcc agcacgaagt ctggagacct ctggcggcag
      601 cctaccaaga acaactggac cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag
      661 tgtgggtccg ccgacaccag actaagaacc tagaacctcg ctggaaagga ccttacacag
      721 tcctgctgac cacccccacc gccctcaaag tagacggcat cgcagcttgg atacacgccg
      781 cccacgtgaa ggctgccgac cccgggggtg gaccatcctc tagactgccg gatctagcta
      841 gttaattaag gatcccagtg tggtggtacg ggaaatcaca agtttgtaca aaaaagcagg
      901 ctccgcggcc gcccccttca ccatgggctc cgtgtccaac cagcagtttg caggtggctg
      961 cgccaaggcg gcagaagagg cgcccgagga ggcgccggag gacgcggccc gggcggcgga
     1021 cgagcctcag ctgctgcacg gtgcgggcat ctgtaagtgg ttcaacgtgc gcatggggtt
     1081 cggcttcctg tccatgaccg cccgcgccgg ggtcgcgctc gaccccccag tggatgtctt
     1141 tgtgcaccag agtaagctgc acatggaagg gttccggagc ttgaaggagg gtgaggcagt
     1201 ggagttcacc tttaagaagt cagccaaggg tctggaatcc atccgtgtca ccggacctgg
     1261 tggagtattc tgtattggga gtgagaggcg gccaaaagga aagagcatgc agaagcgcag
     1321 atcaaaagga gacaggtgct acaactgtgg aggtctagat catcatgcca aggaatgcaa
     1381 gctgccaccc cagcccaaga agtgccactt ctgccagagc atcagccata tggtagcctc
     1441 atgtccgctg aaggcccagc agggccctag tgcacaggga aagccaacct actttcgaga
     1501 ggaagaagaa gaaatccaca gccctaccct gctcccggag gcacagaatt gaaagggtgg
     1561 gcgcgccgac ccagctttct tgtacaaagt ggtgatattc cagctgagcg ccggtcgcta
     1621 ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt
     1681 atttcgcgta aggaaatcca ttatgtacta tttaaactcg agcggccgcc agcacagtgg
     1741 tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     1801 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     1861 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     1921 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     1981 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     2041 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     2101 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     2161 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     2221 caacccctca ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     2281 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     2341 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     2401 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     2461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3121 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3361 cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata tgagtaaact
     3421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3541 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4261 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4321 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4381 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcataatg aaagacccca
     4441 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
     4501 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
     4561 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
     4621 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     4681 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
     4741 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     4801 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     4861 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
     4921 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     4981 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
     5041 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
     5101 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
     5161 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
     5221 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
     5281 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc cccctaatag gagggatatg
     5341 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     5401 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
     5461 tctgtctgac tg
//
