LOCUS       sequence-151868-        6435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of human MEF2A with an HA  tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Han TH, Prywes R
  TITLE     Regulatory role of MEF2D in serum induction of the c-jun promoter.
  JOURNAL   Mol Cell Biol. 1995 Jun;15(6):2907-15.
  PUBMED    7760790
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1995-06"; volume: "15(6)"; pages: "2907-15"
COMMENT     Sequence Label: pCGN-MEF2A
FEATURES             Location/Qualifiers
     source          1..6435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(238..693)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(375..396)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     587..606
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     819..841
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     833..850
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     834..850
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     857..876
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        857..875
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        894..1197
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1198..1401
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1352..1372
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1398..1422
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           1491..1530
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             1560..1586
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1566..1592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          3027..3599
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3731..3750)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3796..3851
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3850..3869)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4205..4340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4446..4463)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4617..5205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4697..4716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5376..6167,6168..6236))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5999..6018
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6237..6341)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6409..6427
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
      241 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      301 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      361 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      421 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      481 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      541 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      601 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      661 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
      721 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      781 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      841 gacggccagt gaattgtaat acgactcact atagggcgaa ttcgagctcg ccccgttaca
      901 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      961 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1021 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1081 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1141 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     1201 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     1261 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     1321 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     1381 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     1441 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgggg gatcttggtg
     1501 gcgtgaaact cccgcacctc ttcggccagc gccttgtaga agcgcgtatg gcttctagct
     1561 atccttatga cgtgcctgac tatgccagcc tgggaggacc ttctagaatt cgagctcggt
     1621 accccatggg gcggaagaaa atacaaatca cacgcataat ggatgaaagg aaccgacagg
     1681 tcacttttac aaagagaaag tttggattaa tgaagaaagc ctatgaactt agtgtgctct
     1741 gtgactgtga aatagcactc atcattttca acagctctaa caaactgttt caatatgcta
     1801 gcactgatat ggacaaagtt cttctcaagt atacagaata taatgaacct catgaaagca
     1861 gaaccaactc ggatattgtt gaggctctga acaagaagga acacagaggg tgcgacagcc
     1921 cagaccctga tacttcatat gtgctaactc cacatacaga agaaaaatat aaaaaaatta
     1981 atgaggaatt tgataatatg atgcggaatc ataaaatcgc acctggtctg ccacctcaga
     2041 acttttcaat gtctgtcaca gttccagtga ccagccccaa tgctttgtcc tacactaacc
     2101 cagggagttc actggtgtcc ccatctttgg cagccagctc aacgttaaca gattcaagca
     2161 tgctctctcc acctcaaacc acattacata gaaatgtgtc tcctggagct cctcagagac
     2221 caccaagtac tggcaatgca ggtgggatgt tgagcactac agacctcaca gtgccaaatg
     2281 gagctggaag cagtccagtg gggaatggat ttgtaaactc aagagcttct ccaaatttga
     2341 ttggagctac tggtgcaaat agcttaggca aagtcatgcc tacaaagtct ccccctccac
     2401 caggtggtgg taatcttgga atgaacagta ggaaaccaga tcttcgagtt gtcatccccc
     2461 cttcaagcaa gggcatgatg cctccactaa acacccaaag gatcagtagt tctcaagcca
     2521 ctcaacctct tgctacccca gtcgtgtctg tgacaacccc aagcttgcct ccgcaaggac
     2581 ttgtgtactc agcaatgccg actgcctaca acactgatta ttcactgacc agcgctgacc
     2641 tgtcagccct tcaaggcttc aactcgccag gaatgctgtc gctgggacag gtgtcggcct
     2701 ggcagcagca ccacctagga caagcagccc tcagctctct tgttgctgga gggcagttat
     2761 ctcagggttc caatttatcc attaatacca accaaaacat cagcatcaag tccgaaccga
     2821 tttcacctcc tcgggatcgt atgaccccat cgggcttcca gcagcagcag cagcagcagc
     2881 agcagccgcc gccaccaccg cagccccagc cacaaccccc gcagccccag ccccgacagg
     2941 aaatgggtcg ctcccctgtg gacagtctga gcagctctag tagctcctat gatggcagtg
     3001 atcgggagga tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
     3061 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
     3121 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
     3181 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
     3241 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
     3301 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
     3361 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata
     3421 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc ctggtcatca
     3481 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact
     3541 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct ttcctacagc
     3601 tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc
     3661 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     3721 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     3781 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     3841 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     3901 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     3961 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     4021 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     4081 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     4141 tcccagtcat agctgtccct cttctcttat ggagatccct cgaggagctt tttgcaaaag
     4201 cctaggcctc caaaaaagcc tcttcactac ttctggaata gctcagaggc cgaggcggcc
     4261 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     4321 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     4381 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4441 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4501 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5041 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5281 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5341 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5401 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5461 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5521 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5581 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5641 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5701 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5761 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5821 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5881 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5941 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6001 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6061 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6121 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6181 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6241 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6301 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6361 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6421 acgaggccct ttcgt
//
