LOCUS       sequence-151869-       15284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, activator]; p35S:Cas9 
            D10A/N863A-Hax3CT, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 15284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pJOG251
FEATURES             Location/Qualifiers
     source          1..15284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    266..290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(333..585)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(605..1156)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1199..1378)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     polyA_signal    1468..1642
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2545..2565)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2578..4092,4093..4095,4096..4161,4162..4167,
                     4168..6651,6652..6654,6655..6681))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(2578..4092,4093..4095,4096..6651,6652..6654,
                     6655..6681))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2578..6681)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6688..6708)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(6805..7150)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6842..6862)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        7263..7365
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7366..8025
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7432..7451)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8367..8672
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8551..8570
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    9014..9038
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10339..10968
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11402..12469
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      12535..12729
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12965..12987)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13073..13213
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13128..13147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13228..13245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13399..13987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13479..13498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14231..15022)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
       61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
      121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
      181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
      241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
      301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg
      361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
      421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
      481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
      541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg
      601 gtcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca
      661 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc
      721 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga
      781 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga
      841 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg
      901 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc
      961 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc
     1021 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt
     1081 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc
     1141 gtcgttctgg gctcatggta gatccccctc gatcgagttg agagtgaata tgagactcta
     1201 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     1261 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     1321 cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg
     1381 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     1441 cttaattctc cgctcatgat tagtagcgat ctggatttta gtactggatt ttggttttag
     1501 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
     1561 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
     1621 ctcacacatt attatggaga aactcgagct tgtcgatcga ctctagctag agaagcttca
     1681 ctgaggcaat agctccatca accatgcgag ctcctcttcg ttgaatgccg ggaaatcatc
     1741 cgctgtccct gcgaaggggt ccgcatcttg ttcccaaacc acggtgctgg acgctaccag
     1801 gtcggcatcc atgggcgtac caggatccag gaggccgccg atcctggtac gcgggcgttt
     1861 tacaccccag ctgaggaggg gcaaatgcaa cgcatcgcgc tgttcgggaa cgcgcacctc
     1921 gaacgattgc tgtgcggagg gaccggtgac agcacgatcc gatcgggacc gtttacggct
     1981 gcttgcccgc gtctgatctc cctcgtgcat tgggctaggc gcatcaaggt cacgctccag
     2041 cgaatcggcg aatgcatgca aagacgcctg atccggcgtt tgagttgaag taggggacgg
     2101 tttggccctt ttcatccctg atgcctggag gatacggtcc caacgctgcg aggctggggg
     2161 gagcgttcca ctgcgggctt cgagttcggt gacgcccact ctgcgaaaga gctgtaacaa
     2221 cccgtgcctg ctcatcccga actgcgtcat ggcgtcatca aatgcttgcg ctgggtggga
     2281 gtggcactgg aaaaaaccca gcacgcgaac cacttgcgcg tggtcggcaa cgcgatggga
     2341 tgtgcgttcg ggaatacggc gattggttct tttgatcaag gccggcgcgt gcggcaatcc
     2401 ctttttcact gcatccagcg caggacgtcc gccgaggcag gccaaggcga cgaggtggtc
     2461 gttggtcaac gcggccaacg ccggatcagg gcgagataac tgggcaacaa tgcttccacc
     2521 gccaccacta ccgccacccg aacccacctt cctcttcttc ttggggtcag ccctgctgtc
     2581 tccaccgagc tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat
     2641 cagtgtggcg tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc
     2701 gaagtacttg aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt
     2761 ttctgcctgc tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc
     2821 gaggttagcg tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc
     2881 atcaaggtag tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcgggaga
     2941 tcctttgagc ttttcatagt ggctggccag atacaagaaa ttaacgtatt tagagggcag
     3001 tgccagctcg ttacctttct gcagctcgcc cgcactagcg agcattcgtt tccggccgtt
     3061 ttcaagctca aagagagagt acttgggaag cttaatgatg aggtcttttt tgacctcttt
     3121 atatcctttc gcctcgagaa agtcgatggg gtttttttcg aagcttgatc gctccatgat
     3181 tgtgatgccc agcagttcct tgacgctttt gagtttttta gacttccctt tctccacttt
     3241 ggccacaacc agtacactgt aagcgactgt aggagaatcg aatccgccgt atttcttggg
     3301 gtcccaatct tttttgcgtg cgatcagctt gtcgctgttc cttttcggga ggatactttc
     3361 cttggagaag cctccggtct gtacttcggt ctttttaacg atgttcacct gcggcatgga
     3421 caggaccttc cggactgtcg cgaaatccct acccttgtcc cacacgattt ctcctgtttc
     3481 tccgtttgtt tcgataagtg gtcgcttccg aatctctcca ttggccagtg taatctcggt
     3541 cttgaaaaaa ttcataatat tgctgtaaaa gaagtactta gcggtggcct tgcctatttc
     3601 ctgctcagac tttgcgatca ttttcctaac atcgtacact ttatagtctc cgtaaacaaa
     3661 ttcagattca agcttgggat attttttgat aagtgcagtg cctaccactg cattcaggta
     3721 ggcatcatgc gcatggtggt aattgttgat ctctctcacc ttataaaact gaaagtcctt
     3781 tctgaaatct gaaaccagct tagacttcag agtaataact ttcacctctc gaatcagttt
     3841 gtcattttca tcgtacttgg tgttcatgcg tgaatcgaga atttgggcca cgtgcttggt
     3901 gatctggcgt gtctcaacaa gctgcctttt gatgaagccg gctttatcca actcagacag
     3961 gccacctcgt tcagccttag tcagattatc gaacttccgt tgtgtgatca gtttggcgtt
     4021 cagcagctgc cgccaataat ttttcatttt cttgacaact tcttctgagg ggacgttatc
     4081 actcttccct ctagctttat cggatcttgt caacacttta ttatcaatag aatcatcttt
     4141 gagaaaagac tggggcacga tatgatccac gtcgtagtcg gagagccgat tgatgtccag
     4201 ttcctgatcc acgtacatgt ccctgccgtt ctgcaggtag tacaggtaga gcttctcatt
     4261 ctgaagctgg gtgttttcaa ctgggtgttc cttaaggatt tgggacccca gttcttttat
     4321 accctcttca atcctcttca tcctttccct actgttcttc tgtcccttct gggtagtttg
     4381 gttctctcgg gccatctcga taacgatatt ctcgggctta tgccttccca ttactttgac
     4441 gagttcatcc acgaccttaa cggtctgcag tattcccttt ttgatagctg ggctacctgc
     4501 aagattagcg atgtgctcgt ggagactgtc cccctggcca gaaacttgtg ctttctggat
     4561 gtcctcctta aaggtgagag agtcatcatg gatcaactgc atgaagttcc ggttggcaaa
     4621 tccatcggac ttaagaaaat ccaggattgt ctttccactc tgcttgtctc ggatcccatt
     4681 gatcagtttt cttgacagcc gcccccatcc tgtatatcgg cgcctcttga gctgtttcat
     4741 gactttgtcg tcgaagagat gagcgtaagt tttcaagcgt tcttcaatca tctccctatc
     4801 ttcaaacaac gtaagggtga ggacaatgtc ctcaagaatg tcctcgttct cctcattgtc
     4861 caggaagtcc ttgtctttaa tgattttcag gagatcgtga tacgttccca gggatgcgtt
     4921 gaagcgatcc tccactccgc tgatttcaac agagtcgaaa cattcaatct ttttgaaata
     4981 atcttctttg agctgtttca cggtaacttt ccggttcgtc ttgaagagga ggtccacgat
     5041 agctttcttc tgctctccag acaggaatgc tggctttctc atcccttctg tgacgtattt
     5101 gaccttggtg agctcgttat aaactgtgaa gtactcgtac agcagagagt gtttaggaag
     5161 caccttttcg ttaggcagat ttttatcaaa gttagtcatc ctttcgatga aggactgggc
     5221 agaggccccc ttatccacga cttcctcgaa gttccaggga gtgatagtct cttctgattt
     5281 gcgagtcatc cacgcgaatc tggaatttcc ccgggcgagg gggcctacat agtagggtat
     5341 ccgaaatgtg aggattttct caatcttttc cctgttatct ttcaaaaagg ggtagaaatc
     5401 ctcttgccgc ctgaggatag cgtgcagttc gcccaggtga atctggtggg ggatgcttcc
     5461 attgtcgaaa gtgcgctgtt tgcgcaacag atcttctctg ttaagcttta ccagcagctc
     5521 ctcggtgccg tccatttttt ccaagatggg cttaataaat ttgtaaaatt cctcctggct
     5581 tgctccgccg tcaatgtatc cggcgtagcc atttttagac tgatcgaaga aaatttcctt
     5641 gtacttctca ggcagttgct gtctgacaag ggccttcagc aaagtcaagt cttggtggtg
     5701 ctcatcatag cgcttgatca tactagcgct cagcggagct ttggtgatct ccgtgttcac
     5761 tcgcagaata tcactcagca gaatggcgtc tgacaggttc tttgccgcca aaaaaaggtc
     5821 tgcgtactgg tcgccgatct gggccagcag attgtcgaga tcatcatcgt aggtgtcttt
     5881 gctcagttga agcttggcat cttcggccag gtcgaagtta gatttaaagt tgggggtcag
     5941 cccgagtgac agggcgataa gattaccaaa caggccgttc ttcttctccc cagggagctg
     6001 tgcgatgagg ttttcgagcc gccgggattt ggacagccta gcgctcagga ttgctttggc
     6061 gtcaactccg gatgcgttga tcgggttctc ttcgaaaagc tgattgtaag tctgaaccag
     6121 ttggataaag agtttgtcga catcgctgtt gtctgggttc aggtccccct cgatgaggaa
     6181 gtgtccccga aatttgatca tatgcgccag cgcgagatag atcaaccgca agtcagcctt
     6241 atcagtactg tctacaagct tcttcctcag atgatatatg gttgggtact tttcatggta
     6301 cgccacctcg tccacgatat tgccaaagat tgggtggcgc tcgtgctttt tatcctcctc
     6361 caccaaaaag gactcctcca gcctatggaa gaaagagtca tccaccttag ccatctcatt
     6421 actaaagatc tcctgcaggt agcagatccg attctttctg cgggtatatc tgcgccgtgc
     6481 tgttcttttg agccgcgtgg cttcggccgt ctccccggag tcgaacagga gggcgccaat
     6541 gaggttcttc tttatgctgt ggcgatcggt attgcccaga actttgaatt ttttgctcgg
     6601 caccttgtac tcgtccgtaa tgacggccca gccgacgctg tttgtgccga tagcgagccc
     6661 aatggagtac ttcttgtcca tgccaccaac cttccgcttc ttcttaggag ccattgtatc
     6721 gataattgta aatgtaattg taatgttgtt tgttgtttgt tgttgttggt aattgttgta
     6781 aaaatgagct cttatactcg agcgtgtcct ctccaaatga aatgaacttc cttatataga
     6841 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc
     6901 acatcaatcc acttgctttg tagacgtggt tggaacctct tctttttcca cgatgctcct
     6961 cgtgggtggg ggtccatctt tgggaccact gtcggcagag agatcttgaa tgatagcctt
     7021 tcctttatcg caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat
     7081 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgaaatta tcctttgttg
     7141 aaaagtctca atagcccttt gatcttctga gactgtatct ttgacatttt tggagtagac
     7201 cagagtgtcg tgctccacca tgttgacctc caagcttgaa ttcttgcaga gacctgagac
     7261 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     7321 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     7381 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     7441 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     7501 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     7561 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     7621 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     7681 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     7741 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     7801 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     7861 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     7921 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     7981 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatcgcg tggatccggc
     8041 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     8101 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     8161 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     8221 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     8281 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     8341 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     8401 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     8461 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     8521 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     8581 cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     8641 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     8701 cggtctcagc ttccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
     8761 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
     8821 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     8881 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg
     8941 catgcgacgt cgagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     9001 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     9061 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     9121 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     9181 atagtgcagt cggcttctga cgttcagtgc agccgtgttc tgaaaacgac atgtcgcaca
     9241 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     9301 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     9361 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     9421 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     9481 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     9541 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     9601 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     9661 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     9721 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     9781 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     9841 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     9901 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     9961 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
    10021 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
    10081 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
    10141 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
    10201 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
    10261 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
    10321 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
    10381 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
    10441 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
    10501 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
    10561 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
    10621 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
    10681 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
    10741 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
    10801 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
    10861 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
    10921 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
    10981 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
    11041 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
    11101 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
    11161 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
    11221 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
    11281 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
    11341 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
    11401 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
    11461 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
    11521 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
    11581 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
    11641 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
    11701 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
    11761 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
    11821 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
    11881 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
    11941 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
    12001 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
    12061 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
    12121 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
    12181 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
    12241 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
    12301 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
    12361 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
    12421 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
    12481 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtgtct ttcctgtgga
    12541 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
    12601 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
    12661 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
    12721 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
    12781 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
    12841 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
    12901 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
    12961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    13021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
    13081 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
    13141 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    13201 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    13261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    13321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    13381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    13441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    13501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    13561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    13621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    13681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    13741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    13801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    13861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    13921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    13981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    14041 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
    14101 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
    14161 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc
    14221 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
    14281 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
    14341 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
    14401 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
    14461 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
    14521 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
    14581 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
    14641 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
    14701 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
    14761 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
    14821 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
    14881 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
    14941 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
    15001 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg
    15061 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
    15121 tggatgcccg aggcatagac tgtaccccaa aaaaacatgt cataacaaga agccatgaaa
    15181 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac
    15241 ggcagttacg ctacttgcat tacagcttac gaaccgaacg aggc
//
