LOCUS       sequence-151870-       16091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, 
            nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 16091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pJOG252
FEATURES             Location/Qualifiers
     source          1..16091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    266..290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(333..585)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(975..1769)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1086..1105)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1696..1715
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1790..1973)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1826..2005)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     polyA_signal    2095..2269
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3172..3192)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3205..4719,4720..4722,4723..4788,4789..4794,
                     4795..7278,7279..7281,7282..7308))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(3205..4719,4720..4722,4723..7278,7279..7281,
                     7282..7308))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3205..7308)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7315..7335)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7432..7777)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7469..7489)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        7981..8083
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8084..8743
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8150..8169)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9089..9394
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9273..9292
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        9432..9507
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9821..9845
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11146..11775
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12209..13276
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      13342..13536
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13772..13794)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13880..14020
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13935..13954
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14035..14052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14206..14794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14286..14305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15038..15829)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
       61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
      121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
      181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
      241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
      301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg
      361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
      421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
      481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
      541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga tcatccgggt
      601 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt
      661 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga tcatattgtc
      721 gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc
      781 gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat ccccgcgctg
      841 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
      901 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
      961 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1021 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1081 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1141 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1201 tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag cctggcgaac
     1261 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1321 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1381 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1441 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1501 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1561 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1621 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     1681 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     1741 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt gcagattatt
     1801 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     1861 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     1921 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     1981 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     2041 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgattag tagcgatctg
     2101 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
     2161 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg
     2221 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt
     2281 cgatcgactc tagctagaga agcttcactg aggcaatagc tccatcaacc atgcgagctc
     2341 ctcttcgttg aatgccggga aatcatccgc tgtccctgcg aaggggtccg catcttgttc
     2401 ccaaaccacg gtgctggacg ctaccaggtc ggcatccatg ggcgtaccag gatccaggag
     2461 gccgccgatc ctggtacgcg ggcgttttac accccagctg aggaggggca aatgcaacgc
     2521 atcgcgctgt tcgggaacgc gcacctcgaa cgattgctgt gcggagggac cggtgacagc
     2581 acgatccgat cgggaccgtt tacggctgct tgcccgcgtc tgatctccct cgtgcattgg
     2641 gctaggcgca tcaaggtcac gctccagcga atcggcgaat gcatgcaaag acgcctgatc
     2701 cggcgtttga gttgaagtag gggacggttt ggcccttttc atccctgatg cctggaggat
     2761 acggtcccaa cgctgcgagg ctggggggag cgttccactg cgggcttcga gttcggtgac
     2821 gcccactctg cgaaagagct gtaacaaccc gtgcctgctc atcccgaact gcgtcatggc
     2881 gtcatcaaat gcttgcgctg ggtgggagtg gcactggaaa aaacccagca cgcgaaccac
     2941 ttgcgcgtgg tcggcaacgc gatgggatgt gcgttcggga atacggcgat tggttctttt
     3001 gatcaaggcc ggcgcgtgcg gcaatccctt tttcactgca tccagcgcag gacgtccgcc
     3061 gaggcaggcc aaggcgacga ggtggtcgtt ggtcaacgcg gccaacgccg gatcagggcg
     3121 agataactgg gcaacaatgc ttccaccgcc accactaccg ccacccgaac ccaccttcct
     3181 cttcttcttg gggtcagccc tgctgtctcc accgagctga gagaggtcga ttcttgtttc
     3241 atagagcccc gtaattgact gatgaatcag tgtggcgtcc aggacctcct ttgtagaggt
     3301 gtaccgcttt ctgtctatgg tggtgtcgaa gtacttgaag gctgcaggcg cgcccaagtt
     3361 ggtcagagta aacaagtgga taatgttttc tgcctgctcc ctgatgggct tatccctgtg
     3421 cttattgtaa gcagaaagca ccttatcgag gttagcgtcg gcgaggatca ctcttttgga
     3481 gaattcgctt atttgctcga tgatctcatc aaggtagtgt ttgtgttgtt ccacgaacag
     3541 ctgcttctgc tcattatctt cgggagatcc tttgagcttt tcatagtggc tggccagata
     3601 caagaaatta acgtatttag agggcagtgc cagctcgtta cctttctgca gctcgcccgc
     3661 actagcgagc attcgtttcc ggccgttttc aagctcaaag agagagtact tgggaagctt
     3721 aatgatgagg tcttttttga cctctttata tcctttcgcc tcgagaaagt cgatggggtt
     3781 tttttcgaag cttgatcgct ccatgattgt gatgcccagc agttccttga cgcttttgag
     3841 ttttttagac ttccctttct ccactttggc cacaaccagt acactgtaag cgactgtagg
     3901 agaatcgaat ccgccgtatt tcttggggtc ccaatctttt ttgcgtgcga tcagcttgtc
     3961 gctgttcctt ttcgggagga tactttcctt ggagaagcct ccggtctgta cttcggtctt
     4021 tttaacgatg ttcacctgcg gcatggacag gaccttccgg actgtcgcga aatccctacc
     4081 cttgtcccac acgatttctc ctgtttctcc gtttgtttcg ataagtggtc gcttccgaat
     4141 ctctccattg gccagtgtaa tctcggtctt gaaaaaattc ataatattgc tgtaaaagaa
     4201 gtacttagcg gtggccttgc ctatttcctg ctcagacttt gcgatcattt tcctaacatc
     4261 gtacacttta tagtctccgt aaacaaattc agattcaagc ttgggatatt ttttgataag
     4321 tgcagtgcct accactgcat tcaggtaggc atcatgcgca tggtggtaat tgttgatctc
     4381 tctcacctta taaaactgaa agtcctttct gaaatctgaa accagcttag acttcagagt
     4441 aataactttc acctctcgaa tcagtttgtc attttcatcg tacttggtgt tcatgcgtga
     4501 atcgagaatt tgggccacgt gcttggtgat ctggcgtgtc tcaacaagct gccttttgat
     4561 gaagccggct ttatccaact cagacaggcc acctcgttca gccttagtca gattatcgaa
     4621 cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt tcattttctt
     4681 gacaacttct tctgagggga cgttatcact cttccctcta gctttatcgg atcttgtcaa
     4741 cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatat gatccacgtc
     4801 gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc tgccgttctg
     4861 caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg ggtgttcctt
     4921 aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc tttccctact
     4981 gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa cgatattctc
     5041 gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg tctgcagtat
     5101 tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgga gactgtcccc
     5161 ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt catcatggat
     5221 caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca ggattgtctt
     5281 tccactctgc ttgtctcgga tcccattgat cagttttctt gacagccgcc cccatcctgt
     5341 atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag cgtaagtttt
     5401 caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagga caatgtcctc
     5461 aagaatgtcc tcgttctcct cattgtccag gaagtccttg tctttaatga ttttcaggag
     5521 atcgtgatac gttcccaggg atgcgttgaa gcgatcctcc actccgctga tttcaacaga
     5581 gtcgaaacat tcaatctttt tgaaataatc ttctttgagc tgtttcacgg taactttccg
     5641 gttcgtcttg aagaggaggt ccacgatagc tttcttctgc tctccagaca ggaatgctgg
     5701 ctttctcatc ccttctgtga cgtatttgac cttggtgagc tcgttataaa ctgtgaagta
     5761 ctcgtacagc agagagtgtt taggaagcac cttttcgtta ggcagatttt tatcaaagtt
     5821 agtcatcctt tcgatgaagg actgggcaga ggccccctta tccacgactt cctcgaagtt
     5881 ccagggagtg atagtctctt ctgatttgcg agtcatccac gcgaatctgg aatttccccg
     5941 ggcgaggggg cctacatagt agggtatccg aaatgtgagg attttctcaa tcttttccct
     6001 gttatctttc aaaaaggggt agaaatcctc ttgccgcctg aggatagcgt gcagttcgcc
     6061 caggtgaatc tggtggggga tgcttccatt gtcgaaagtg cgctgtttgc gcaacagatc
     6121 ttctctgtta agctttacca gcagctcctc ggtgccgtcc attttttcca agatgggctt
     6181 aataaatttg taaaattcct cctggcttgc tccgccgtca atgtatccgg cgtagccatt
     6241 tttagactga tcgaagaaaa tttccttgta cttctcaggc agttgctgtc tgacaagggc
     6301 cttcagcaaa gtcaagtctt ggtggtgctc atcatagcgc ttgatcatac tagcgctcag
     6361 cggagctttg gtgatctccg tgttcactcg cagaatatca ctcagcagaa tggcgtctga
     6421 caggttcttt gccgccaaaa aaaggtctgc gtactggtcg ccgatctggg ccagcagatt
     6481 gtcgagatca tcatcgtagg tgtctttgct cagttgaagc ttggcatctt cggccaggtc
     6541 gaagttagat ttaaagttgg gggtcagccc gagtgacagg gcgataagat taccaaacag
     6601 gccgttcttc ttctccccag ggagctgtgc gatgaggttt tcgagccgcc gggatttgga
     6661 cagcctagcg ctcaggattg ctttggcgtc aactccggat gcgttgatcg ggttctcttc
     6721 gaaaagctga ttgtaagtct gaaccagttg gataaagagt ttgtcgacat cgctgttgtc
     6781 tgggttcagg tccccctcga tgaggaagtg tccccgaaat ttgatcatat gcgccagcgc
     6841 gagatagatc aaccgcaagt cagccttatc agtactgtct acaagcttct tcctcagatg
     6901 atatatggtt gggtactttt catggtacgc cacctcgtcc acgatattgc caaagattgg
     6961 gtggcgctcg tgctttttat cctcctccac caaaaaggac tcctccagcc tatggaagaa
     7021 agagtcatcc accttagcca tctcattact aaagatctcc tgcaggtagc agatccgatt
     7081 ctttctgcgg gtatatctgc gccgtgctgt tcttttgagc cgcgtggctt cggccgtctc
     7141 cccggagtcg aacaggaggg cgccaatgag gttcttcttt atgctgtggc gatcggtatt
     7201 gcccagaact ttgaattttt tgctcggcac cttgtactcg tccgtaatga cggcccagcc
     7261 gacgctgttt gtgccgatag cgagcccaat ggagtacttc ttgtccatgc caccaacctt
     7321 ccgcttcttc ttaggagcca ttgtatcgat aattgtaaat gtaattgtaa tgttgtttgt
     7381 tgtttgttgt tgttggtaat tgttgtaaaa atgagctctt atactcgagc gtgtcctctc
     7441 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     7501 gtcatccctt acgtcagtgg agatgtcaca tcaatccact tgctttgtag acgtggttgg
     7561 aacctcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     7621 ggcagagaga tcttgaatga tagcctttcc tttatcgcaa tgatggcatt tgtaggagcc
     7681 accttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat ggaatccgag
     7741 gaggtttccc gaaattatcc tttgttgaaa agtctcaata gccctttgat cttctgagac
     7801 tgtatctttg acatttttgg agtagaccag agtgtcgtgc tccaccatgt tgacctccaa
     7861 gcttgaattc ttgctaagtt gaaaacaatc ttcaaaagtc ccacatcgct tagataagaa
     7921 aacgaagctg agtttatata cagctagagt cgaagtagtg attgaagtct tctgagacgt
     7981 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
     8041 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     8101 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     8161 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     8221 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     8281 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     8341 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     8401 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     8461 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     8521 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     8581 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     8641 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     8701 tgaattacaa cagtactgcg atgagtggca ggggggggcg taaacgctcg cgtggatccg
     8761 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     8821 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     8881 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     8941 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     9001 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     9061 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     9121 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     9181 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     9241 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     9301 gccggtttcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     9361 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     9421 gtcgaagact tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     9481 cttgaaaaag tggcaccgag tcggtgcttt tttttgcttc catgctagag tccgcaaaaa
     9541 tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt
     9601 agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     9661 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     9721 taaaaccaaa atccagtgac ctgcaggcat gcgacgtcga gcttagcttg agcttggatc
     9781 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     9841 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     9901 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     9961 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
    10021 cgtgttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
    10081 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
    10141 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
    10201 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
    10261 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
    10321 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
    10381 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
    10441 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
    10501 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
    10561 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
    10621 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
    10681 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
    10741 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
    10801 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
    10861 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
    10921 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
    10981 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
    11041 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
    11101 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
    11161 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
    11221 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
    11281 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
    11341 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
    11401 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
    11461 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
    11521 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
    11581 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
    11641 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
    11701 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
    11761 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
    11821 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
    11881 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
    11941 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
    12001 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
    12061 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
    12121 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
    12181 acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg
    12241 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
    12301 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
    12361 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
    12421 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
    12481 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
    12541 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
    12601 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
    12661 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
    12721 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
    12781 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
    12841 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
    12901 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
    12961 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
    13021 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
    13081 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
    13141 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
    13201 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
    13261 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
    13321 aggtcgaaaa ggtgtctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
    13381 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
    13441 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
    13501 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
    13561 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
    13621 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
    13681 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
    13741 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    13801 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    13861 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
    13921 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    13981 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
    14041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    14101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    14161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    14221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    14281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    14341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    14401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    14461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    14521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    14581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    14641 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    14701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    14761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    14821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc atgatatatc
    14881 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
    14941 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa tcgcttgagt taacgccggc
    15001 gaagcggcgt cggcttgaac gaatttctag ctagacatta tttgccgact accttggtga
    15061 tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat
    15121 cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg
    15181 gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg
    15241 cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg
    15301 gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa
    15361 ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt
    15421 ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa
    15481 tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa
    15541 tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag
    15601 gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc
    15661 ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg
    15721 cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa
    15781 ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttcccccatg atgtttaact
    15841 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc
    15901 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa
    15961 aacatgtcat aacaagaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
    16021 gtcaaggttc tggaccagtt gcgtgacggc agttacgcta cttgcattac agcttacgaa
    16081 ccgaacgagg c
//
