LOCUS       sequence-151871-        6836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of mouse MEF2D with an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6836)
  TITLE     Prywes Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCGN-MEF2D
FEATURES             Location/Qualifiers
     source          1..6836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(238..693)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(375..396)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     587..606
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     819..841
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     833..850
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     834..850
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     857..876
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        857..875
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        894..1197
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1198..1401
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1352..1372
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1398..1422
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           1491..1530
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             1560..1586
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1566..1592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          3428..4000
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4132..4151)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4197..4252
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4251..4270)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4606..4741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4847..4864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5018..5606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5098..5117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5777..6568,6569..6637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6400..6419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6638..6742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6810..6828
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
      241 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      301 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      361 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      421 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      481 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      541 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      601 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      661 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
      721 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      781 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      841 gacggccagt gaattgtaat acgactcact atagggcgaa ttcgagctcg ccccgttaca
      901 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      961 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1021 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1081 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1141 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     1201 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     1261 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     1321 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     1381 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     1441 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgggg gatcttggtg
     1501 gcgtgaaact cccgcacctc ttcggccagc gccttgtaga agcgcgtatg gcttctagct
     1561 atccttatga cgtgcctgac tatgccagcc tgggaggacc ttctagaatg gggaggaaaa
     1621 agattcagat ccagcgaatc actgatgaac ggaaccgcca ggtgaccttc accaagcgga
     1681 agtttggact gatgaagaag gcctacgagc tgagtgtgct gtgcgactgc gagatcgcgc
     1741 tcatcatctt caaccactcc aacaagctgt tccagtatgc cagcaccgac atggacaagg
     1801 tgctgctcaa gtacaccgag tacaacgagc cacacgagag ccgcaccaat gctgacatca
     1861 tcgagaccct gaggaagaag ggtttcaacg gctgtgacag cccagagccg gatggggagg
     1921 actcactgga gcagagcccc ctgctggagg acaagtaccg gcgggccagt gaggagctgg
     1981 atgggctctt caggcgctat gggtcatctg ttccggcccc caactttgcc atgcctgtca
     2041 cagtgcccgt gtccaatcag agctccatgc agttcagcaa tccaagtagc tctctggtca
     2101 ctccttccct ggtgacatca tcccttacgg acccacggct cctgtccccc cagcagccag
     2161 cactacagag aaacagtgtt tctccaggct tgccccagcg gcctgctagt gcaggagcca
     2221 tgctgggtgg agacctcaac agtgctaatg gagcctgccc cagccccgtt gggaatggct
     2281 atgtcagtgc ccgagcttcc cctggcctcc tccctgtggc caatggcaac agcctaaaca
     2341 aagtcatccc tgccaagtct ccgcccccac ccacccacaa cacccagctt ggagccccca
     2401 gccgcaagcc tgatctgcgg gtcatcactt cccagggagg caaagggtta atgcatcatt
     2461 tgactgggga ccatttagat ctgaacaatg cccagcgcct tggggtctcc cagtctaccc
     2521 actcgctcac caccccagtg gtttccgtgg caacaccaag tttactcagc cagggcctcc
     2581 ccttctcctc catgcccact gcctacaaca cagattacca gctgcccagt gcagagctat
     2641 cctccttacc agccttcagt tcacctgcag ggctggcact aggcaatgtc accgcctggc
     2701 agcagcccca gccgccccag cagccacaac cgccacaacc gccacagtca cagccacagc
     2761 caccacagcc acagccacag cagccacctc agcaacagcc ccacttggtc cccgtttctc
     2821 tcagcaacct catccctggc agccccttgc ctcacgtggg tgctgctctc acagtcacta
     2881 cccaccccca catcagcatc aagtcagaac cagtgtcccc aagtcgtgaa cgcagccctg
     2941 cacctcctcc accagctgtg ttcccagctg cccgccctga gcctggcgaa ggtctcagca
     3001 gcccagctgg aggatcctat gagaccgggg accgggatga tggacggggg gactttgggc
     3061 ccacactagg cctgctgcgc ccagccccag agcctgaggc tgagggctca gctgtgaaga
     3121 ggatgcggct ggatacttgg acattaaagt gatggttccc actccctcct ttcagcctcc
     3181 ctgatgaaga gttgacaatc tcaccgccca ccccctcctt atcctgggct cctcccgctc
     3241 gaccccactt cctttcttct gctccatgtc ctgttgatgg ttacatttgt gtataattat
     3301 attattatta ttgttattat tattatattt ttaaattcga attctgcaga tatccatcac
     3361 actggcggcc gctcgagcat gcatctagaa ttcgagctcg gtacccgggg atcctgagaa
     3421 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
     3481 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
     3541 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
     3601 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
     3661 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
     3721 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
     3781 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
     3841 aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg
     3901 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
     3961 ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt
     4021 tgttgtgctg tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca
     4081 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     4141 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     4201 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     4261 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     4321 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     4381 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     4441 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     4501 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     4561 tcttctctta tggagatccc tcgaggagct ttttgcaaaa gcctaggcct ccaaaaaagc
     4621 ctcttcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     4681 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     4741 tgggcggagt taggggcggg actatggttg ctgactaatt gctgcattaa tgaatcggcc
     4801 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6721 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     6781 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
