LOCUS       sequence-151876-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone to clone single or  paired sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pDECKO_mCherry
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    301..418
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(474..549)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc
       61 gcgccacctt ctctaggcac cggatcaatt gccgacccct ccccccaact tctcggggac
      121 tgtgggcgat gtgcgctctg cccactgacg ggcaccggag ccaattccca ctcctttcaa
      181 gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta tccatctttg
      241 cacccgggcc ccctcgagcc ggcgccaaag tggatctctg ctgtccctgt aataaacccg
      301 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
      361 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
      421 gtggatgaat actgccattt gtctcaagat ctagttacgc caagcttaaa aaagcaccga
      481 ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag
      541 ctctaaaaca gagacgtaca aaaaagagca agaagctaaa aaagatttaa aaattatttt
      601 tagcgcagtt aatggaacag gaactaaatt taccccaaaa atattacgtg aatcaggata
      661 taacgttatt gaggttgaag agcatgcatt tgaagatgaa acatttaaaa atgttgtaaa
      721 tccaaatcca gaatttgatc ctgcatgaaa aataccgctt gaatatggta ttaaacatga
      781 tgcagatatt attattatga atgacccaga tgctgacaga tttggaatgg caataaaaca
      841 tgatggtcat tttgtaagat tagatggaaa tcaaacagga ccaattttaa ttgattgaaa
      901 attatcaaat ctaaaacgct taaatagcat tccaaaaaat ccggctctat attcaagttt
      961 tgtaacaagt ga
//
