LOCUS       sequence-151880-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control plasmid (paired
            inst GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pDECKO-mCherry GFP
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        11..259
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     11..31
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     182..201
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        281..356
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        363..577
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     498..517
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        606..681
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    737..854
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
       61 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
      121 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      181 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      241 gtggaaagga cgaaacaccg gagctggacg gcgacgtaaa gttttagagc tagaaatagc
      301 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
      361 ttgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg
      421 aacacccagc gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc
      481 cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt
      541 tggatttggg agtcttataa gttctgtatg agaccactct ttccccagaa cacccccatc
      601 ggcgagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      661 aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg
      721 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      781 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      841 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
      901 agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
      961 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     1021 g
//
