LOCUS       sequence-151882-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes chloramphenicol resistance gene for 
            positive selection and a toxin gene (relE) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-242
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    130..177
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(255..282)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(374..460)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        550..654
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
       61 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaggcgcgc
      121 catttaaatg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgaa
      181 gcagctccag cctacacaat cgctcaagac gtgtaatgct gcaatctgca tgcaagcttg
      241 gcactggcca cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg
      301 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat
      361 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      421 gaaaggccca gtctttcgac tgagcctttc attttatttg atgcctggca gttccctact
      481 ctcgcatggg gagaccccac actaccatcg ggggccatcg atgcaggtgg cacttttcgg
      541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcatggcat
      781 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      841 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      901 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      961 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1021 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1081 aggaccactt ctgcgctcgg cccttccggc tgg
//
