LOCUS       sequence-151892-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mycobacterial dual reporter plasmid for fluorescence dilution. 
            Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635
            under control of theophylline - inducible riboswitch)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Mouton JM, Helaine S, Holden DW, Sampson SL
  TITLE     Elucidating population-wide mycobacterial replication dynamics at 
            the single-cell level.
  JOURNAL   Microbiology. 2016 Mar 30. doi: 10.1099/mic.0.000288.
  PUBMED    27027532
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2016 Mar 30. doi: 10.1099/mic.0.000288."
COMMENT     Sequence Label: pTiGc
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
       61 actgcagaat tcgaagcttg atatcgaatt cctgcagccc gggggatccg caattgtctt
      121 ggccattgcg aagtgattcc tccggatcgg ggatgaaacg ggggtcaccg ggtgacggca
      181 accccgctac ttacgctggc cgggcgcagt gcccgcgacg gacggctcaa gttgtcctcg
      241 ctgccactcg ctgcgacgac gggcctggcc tcaccgtccc gacctagcac tcaccggtcg
      301 cgagtgccaa cgttattctt agcactcgcc tatgccgagt gcaagaagcc ccgcaccggg
      361 tcatgcccct cgttcgacct tgtcctcggc cctccgatcc gggtgagtat gttcggccca
      421 tgaccgccaa cgacaacaag acccgtaaat ggtcggccgc agacgtcccc gatcaaagcg
      481 ggcgcgttgt ggtcaccgtc tagagcggcc gcgccacgga tgccaccaca ggcactacaa
      541 cggagttcgc cacgtacatc accacaacca ccgattctgg cggtgagctc cccgatattc
      601 agcggaaatg gcttggtatc gaccaagatt cgtagaaccc cgtctcgtct ggctggtatt
      661 caaaacgggc gcaacgaaac acgcaacgag acaggcatgg cccaaaccag aaaactagcg
      721 tctaccagga cttttacctg tccgacccgt tgcaacggaa ccccccacgg aacccccgcg
      781 acacccgctc cccaattgcg ttagaacagc ggtggattgt cggcttcgtt gtgggccttt
      841 tgagccgctt cctgttctgc cgcacgctct ttcctcgccc gatagccgag tcgcttaacg
      901 gtgtccagat gcagcccgaa atgtttggcc gtttgcggcc aagagtggcc ctcgtcgtcg
      961 tgataggcgc ggatgcgttc gcggcg
//
