LOCUS       sequence-151901-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a 
            barcode landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     Zador Lab MAPseq plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: jk100
            pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandin...
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             179..226
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             263..292
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             299..844
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 aaagcatctc tacggtggtc ctaaatagtc agcatagtac atttcatctg actaatacca
       61 caacaccacc acctctagag ccaccatgcc accttctact agtcttttgt tgcttgctgc
      121 acttctcccc ttcgctcttc ccgcttcaga ttggaagaca ggagaagtca ccgcttcccg
      181 cgaccacatg gtgctgcacg aatatgtgaa cgctgctggc atcacaggcg gtggcggatc
      241 tggcggcggt ggtagcgtcg acgaacaaaa actcatctca gaagaggatc tacaattcat
      301 ggacaaagac tgcgaaatga agcgcaccac cctggatagc cctctgggca agctggaact
      361 gtctgggtgc gaacagggcc tgcaccgtat catcttcctg ggcaaaggaa catctgccgc
      421 cgacgccgtg gaagtgcctg ccccagccgc cgtgctgggc ggaccagagc cactgatcca
      481 ggccaccgcc tggctcaacg cctactttca ccagcctgag gccatcgagg agttccctgt
      541 gccagccctg caccacccag tgttccagca ggagagcttt acccgccagg tgctgtggaa
      601 actgctgaaa gtggtgaagt tcggagaggt catcagcgag agccacctgg ccgccctggt
      661 gggcaatccc gccgccaccg ccgccgtgaa caccgccctg gacggaaatc ccgtgcccat
      721 tctgatcccc tgccaccggg tggtgcaggg cgacagcgac gtggggccct acctgggcgg
      781 gctcgccgtg aaagagtggc tgctggccca cgagggccac agactgggca agcctgggct
      841 gggtggcggt ggcggatctg tcgactttca gaaagctagc tctattgttt ataagaagga
      901 gggggaa
//
