LOCUS       sequence-151904-         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1d - Step1 vector d (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1d
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(80..99)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    124..172
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgggt ttaaacctaa tgatgcctga
       61 tgtttttatc gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
      121 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
      181 ggggttcggt cccagggctg gcactctgtc aagtgaacgc tctatggtct aaagatttaa
      241 atctatatct caatcggggt tacgtcgata ccccaccgag accccattgg gaccaatacg
      301 cccgcgtttc ttccttttcc ccaccccaac ccccaagttc gggtgaaggc ccagggctcg
      361 cagccaacgt cggggcggca agccctgcca tagccactac gggttaatta aaaacggttc
      421 acatagctta gtttaaacag gagatccgaa ccagataagt gaaatctagt tccaaactat
//
