LOCUS       sequence-151906-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Ramalho JJ, Sepers JJ, Nicolle O, Schmidt R, Cravo J, Michaux G, 
            Boxem M
  TITLE     C-terminal phosphorylation modulates ERM-1 localization and dynamics
            to control cortical actin organization and support lumen formation 
            during Caenorhabditiselegans development.
  JOURNAL   Development. 2020 Jul 22;147(14):dev188011. doi: 10.1242/dev.188011.
  PUBMED    32586975
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-07-22"; volume: "147(14):dev188011. 
            doi"; pages: " 10.1242/dev.188011"
COMMENT     Sequence Label: pJJR82
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(525..830)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(627..646)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtaccgaact gtttaaactt accttgagct cgatacggtt gacgagggtg tctccctcga
       61 acttgacctc ggcacgggtc ttgtagtttc cgtcgtcctg aaaatttaaa taatcagggt
      121 tagttagtat taattaatgt ttaaacttac cttgaagaag atggtacgct cttggacgta
      181 tccctctggc atggcggact tgaagaagtc gtgacgcttc atgtggtctg ggtaacggga
      241 gaagcattgg actccgtaag tgaaggtggt gacgagggtt ggccatggga ctgggagctt
      301 tccggtggtg cagatgaact tgagggtgag ctttccgtag gtggcgtctc cctctccctc
      361 tcctgagacg gagaacttgt gtccgttgac gtctccgtcg agctcgacga ggattgggac
      421 gactccggtg aagagctcct ctcccttgga catcgatgct cctgaggctc ccgatgctcc
      481 ctaggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
      541 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
      601 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
      661 ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag acgtgcactg
      721 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
      781 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
      841 cctgttctcg tcggcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
      901 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
      961 tgtgc
//
