LOCUS       sequence-151910-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a 
            barcode landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     Zador Lab MAPseq plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: jk100
            pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandin...
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(316..381)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      378..387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(445..510)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      507..516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(574..639)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      636..645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(703..768)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      765..774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtggtggtgt tgtggtatta gtcagatgaa atgtactatg ctgactattt aggaccaccg
       61 tagagatgct ttaaggcctg catgcgccgc ctatacgtag tactctttat ccttgttttt
      121 cttgtttttg tttgctgatt tggcgctgga gggctgtttc tctttaacca cggcgccatt
      181 actctgagca ctgttactga tatagttcct agattcgtca acgtggtaag cgctcggtgg
      241 ggtggctaga ccgccagctc cggctccagc tccagcgcca gctccggctc cgtcgagcgg
      301 tggctcgagc ggtgggtttg cagctttcca ttgagcttgt ttctcagcgc gacgctcacg
      361 tcgtcgtgtt tgtgcgtcca tggtggctag accgccagct ccggctccag ctccagcgcc
      421 agctccggct ccgtcgagcg gtgggtttgc agctttccat tgagcttgtt tctcagcgcg
      481 acgctcacgt cgtcgtgttt gtgcgtccat ggtggctaga ccgccagctc cggctccagc
      541 tccagcgcca gctccggctc cgtcgagcgg tgggtttgca gctttccatt gagcttgttt
      601 ctcagcgcga cgctcacgtc gtcgtgtttg tgcgtccatg gtggctagac cgccagctcc
      661 ggctccagct ccagcgccag ctccggctcc gtcgagcggt gggtttgcag ctttccattg
      721 agcttgtttc tcagcgcgac gctcacgtcg tcgtgtttgt gcgtccatgg tggctagacc
      781 gccagctccg gctccagctc cagcgccagc tccggctccg tcgagcggtg gagcgctaga
      841 tccctcatcc ctgttgcgat acttgtagcg tacgcagaag aagatgccca ggccgataaa
      901 cagaagcagg ccggcgactc ccccgagtac gatcagggcc atc
//
