LOCUS       sequence-151916-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow sequence of interest 
            to be fused to N-terminal double V5 epitope tag. 
            attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP4xV5_ccdB
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(275..316)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(323..364)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtcatat gaccagtaga gtcaagacca agcaagggat
      301 tgggaatagg cttaccagca ccagtactgt ccaagcctaa caaagggtta ggaataggtt
      361 tacccataga ctcgaggcca atttaacaaa gcgactataa gtcagaaagt gagaatgaga
      421 ttgaaataat taattcaact tattcaattg atgatatgcc aggattcctc ttcctttata
      481 tatcttcaat gtttcgatta acaggtccat tatccggttt ttagtttcgc tgcacattgc
      541 cgaatgacaa tcggattcct ttaagctttg cttttgccct gttttctctc cccagtaatc
      601 gttgggtgac tgaaccatcc agtagccgag agttacgttt ttcctgattt aataaatatc
      661 catatctatt tgtctcgtgg tgttttacag caactaaccg aaattactat tttgcatcat
      721 ccaaccataa agagacaaca ataattctag aaaa
//
