LOCUS       sequence-151917-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow sequence of interest 
            to be fused to N-terminal double V5 epitope tag. 
            attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP4xV5_ccdB
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcttgtgat attgacgaaa ctttttgaca tctaatttat tctgttccaa caccaatgtt
       61 tataaccaag ttttatcttg tttgtctaca tggtatttta cattcatcta catacatctt
      121 tcattggctt tgtacatagt tatcattaca agtctaaaaa aattcactct tttcttattc
      181 aatgtcaatc caagagaaaa gattgtggta atgttgtagg agcatgttta ataaattact
      241 atagcaaatt actttttatt cccaaggtgt ttatctataa tagttaatat tttagtcgct
      301 acataaaatt ttaccaaaga gtacttgtat actaattcta aatgccttct gacataaaac
      361 gcctaggaaa acaaacgcaa acaaggcatc gactttttca ataaccaacc aaaaaaattt
      421 tacattagtc tttttttaat gctgagaaag tctttgctga tatgccttcc aaccagcttc
      481 tctatatctc ttggcttcga caacaggatt acgaccagct ccatagactc cacgaccaac
      541 aatgatgata tcgctaccgc agtttacaat cacttcttca ggagtacgat attgctgtcc
      601 cagcccgtct cctttaacat ccaagccgat accaggggac atagttatgt agtcgctttg
      661 aaggttagga aatcgacgac cagctataaa gccaaagcaa aaatcggtat gcttctcaaa
      721 ccattctaag gttttctctg tgtaggaacc agtagccaaa gagcctttgg aagacatttc
      781 agccaaaagc aagagaccac gtcccaaagg taaaccaact tctttgaggc cttgtataat
      841 accctcgcct ggcactgtat ggcaatt
//
