LOCUS       sequence-151919-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow sequence of interest 
            to be fused to N-terminal double V5 epitope tag. 
            attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP41xV5_ccdB
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(275..316)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(323..364)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtcatat gaccagtaga gtcaagacca agcaagggat
      301 tgggaatagg cttaccagca ccagtactgt ccaagcctaa caaagggtta ggaataggtt
      361 tacccataga ctcgaggtat gatttaacaa agcgactata agtcagaaag tgagaatgag
      421 attgaaataa ttaattcaac ttattcaatt gatgatatgc caggattcct cttcctttat
      481 cttcaatgtt tcgattaaca ggtccattat ccggttttta gtttcgctgc acattgccga
      541 atgacaatcg gattccttta agctttgctt ttgccctgtt ttctctcccc agtaatcgtt
      601 gggtgactga accatccagt agccgagagt tacgtttttc ctgatttaat aaatatccat
      661 atctatttgt ctcgtggtgt tttacagcaa ctaaccgaaa ttactatttt gcatcatcca
      721 accataaaga gacaacaata attctagaaa acatataaca agtgtttttt tctatttacg
      781 tctcatacgc aaagtccact ttaccatgtt ttggaaactt
//
