LOCUS       sequence-151923-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow sequence of interest 
            to be fused to N-terminal double V5 epitope tag. 
            attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP42xV5_ccdB
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtttataacc aagttttatc ttgtttgtct acatggtatt ttacattcat ctacatacat
       61 ctttcattgg ctttgtacat agttatcatt acaagtctaa aaaaattcac tcttttctta
      121 ttcaatgtca atccaagaga aaagattgtg gtaatgttgt aggagcatgt ttaataaatt
      181 actatagcaa attacttttt attcccaagg tgtttatcta taatagttaa tattttagtc
      241 gctacataaa attttaccaa agagtacttg tatactaatt ctaaatgcct tctgacataa
      301 aacgcctagg aaaacaaacg caaacaaggc atcgactttt tcaataacca accaaaaaaa
      361 ttttacatta gtcttttttt aatgctgaga aagtctttgc tgatatgcct tccaaccagc
      421 ttctctatat ctcttggctt cgacaacagg attacgacca gctccataga ctccacgacc
      481 aacaatgatg atatcgctac cgcagtttac aatcacttct tcaggagtac gatattgctg
      541 tcccagcccg tctcctttaa catccaagcc gataccaggg gacatagtta tgtagtcgct
      601 ttgaaggtta ggaaatcgac gaccagctat aaagccaaag caaaaatcgg tatgcttctc
      661 aaaccattct aaggttttct ctgtgtagga accagtagcc aaagagcctt tggaagacat
      721 ttcagcc
//
