LOCUS       sequence-151924-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow sequence of interest 
            to be fused to N-terminal double V5 epitope tag. 
            attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP81xV5_ccdB
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..270)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(280..321)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(328..369)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca gcttttttgt acaaacttgt catatgacca gtagagtcaa gaccaagcaa
      301 gggattggga ataggcttac cagcaccagt actgtccaag cctaacaaag ggttaggaat
      361 aggtttaccc atagactcga ggtatgattt aacaaagcga ctataagtca gaaagtgaga
      421 atgagattga aataattaat tcaacttatt caattgatga tatgccagga ttcctcttcc
      481 atcttcaatg tttcgattaa caggtccatt atccggtttt tagtttcgct gcacattgcc
      541 gaatgacaat cggattcctt taagctttgc ttttgccctg ttttctctcc ccagtaatcg
      601 ttgggtgact gaaccatcca gtagccgaga gttacgtttt tcctgattta ataaatatcc
      661 atatctattt gtctcgtggt gttttacagc aactaaccga aattactatt ttgcatcatc
      721 caaccataaa gagacaacaa taattctaga aaacatataa caagtgtttt tttctattta
      781 cgtctcatac gcaaagtcca ctttaccatg ttttggaaac ttgtaccc
//
