LOCUS       sequence-151925-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow sequence of interest 
            to be fused to N-terminal double V5 epitope tag. 
            attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP81xV5_ccdB
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcttgtcga tcgactacgt cgttaaggcc gtttctgaca gagtaaaatt cttgagggaa
       61 ctttcaccat tatgggaaat ggttcaagaa ggtattgact taaactccat caaatggtca
      121 ggtcattgag tgttttttat ttgttgtatt tttttttttt tagagaaaat cctccaatat
      181 ataaattagg aatcatagtt tcatgatttt ctgttacacc taactttttg tgtggtgccc
      241 tcctccttgt caatattaat gttaaagtgc aattcttttt ccttatcacg ttgagccatt
      301 agtatcaatt tgcttacctg tattccttta catcctcctt tttctccttc ttgataaatg
      361 tatgtagatt gcgtatatag tttcgtctac cctatgaaca tattccattt tgtaatttcg
      421 tgtcgtttct attatgaatt tcatttataa agtttatgta caaatatcat aaaaaaagag
      481 aatcttttta agcaaggatt ttcttaactt cttcggcgac agcatcaccg acttcggtgg
      541 tactgttgga accacctaaa tcaccagttc tgatacctgc atccaaaacc tttttaactg
      601 catcttcaat ggccttacct tcttcaggca agttcaatga caatttcaac atcattgcag
      661 cagacaagat agtggcgata gggttgacct tattctttgg caaatctgga gcagaaccgt
      721 ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc aaggacgcag
      781 atggcaacaa acccaaggaa cctgggataa cggaggcttc atcggagatg atatcaccaa
      841 acatgttgct ggtgattata ataccattta gg
//
