LOCUS       sequence-151926-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow sequence of interest 
            to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2
            cassette, nmt1** (AT) promoter, ura4+ selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP82xV5_ccdB
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(275..316)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(323..364)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtcatat gaccagtaga gtcaagacca agcaagggat
      301 tgggaatagg cttaccagca ccagtactgt ccaagcctaa caaagggtta ggaataggtt
      361 tacccataga ctcgaggtat gatttaacaa agcgactata agtcagaaag tgagaatgag
      421 attgaaataa ttaattcaac ttattcaatt gatgatatgc caggattcct cttccatctt
      481 caatgtttcg attaacaggt ccattatccg gtttttagtt tcgctgcaca ttgccgaatg
      541 acaatcggat tcctttaagc tttgcttttg ccctgttttc tctccccagt aatcgttggg
      601 tgactgaacc atccagtagc cgagagttac gtttttcctg atttaataaa tatccatatc
      661 tatttgtctc gtggtgtttt acagcaacta accgaaatta ctattttgca tcatccaacc
      721 ataaagagac aacaataatt ctagaaaaca tataacaagt gtttttttct atttacgtct
      781 catacgcaaa gtccacttta ccatgttttg g
//
