LOCUS       sequence-151931-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow N-terminal tag(s) to 
            be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 
            (ATATATAAA) promoter, LEU2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP3xN_ccdB
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt catatgacta gtctcgaggc caatttaaca aagcgactat
      301 aagtcagaaa gtgagaatga gattgaaata attaattcaa cttattcaat tgatgatatg
      361 ccaggattcc tcttccttta tatatcttca atgtttcgat taacaggtcc attatccggt
      421 ttttagtttc gctgcacatt gccgaatgac aatcggattc ctttaagctt tgcttttgcc
      481 ctgttttctc tccccagtaa tcgttgggtg actgaaccat ccagtagccg agagttacgt
      541 ttttcctgat ttaataaata tccatatcta tttgtctcgt ggtgttttac agcaactaac
      601 cgaaattact attttgcatc atccaaccat aaagagacaa caataattct agaaaacata
      661 taacaagtgt ttttttctat ttacgtctca tacgcaaagt ccactttacc atgttttgga
      721 aacttgtacc caaccattca tttttcgatt ttgtaatgag gctcttttct tctaacaatc
      781 attgaggtca ttatcatgtg tcctcaccta ttgtttaaat tgaggcttgc gctattccc
//
