LOCUS       sequence-151953-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow N-terminal tag(s) to 
            be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* 
            (ATAAA) promoter, LEU2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP41xN_ccdB
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgttatat gactagtctc gaggtatgat ttaacaaagc
      301 gactataagt cagaaagtga gaatgagatt gaaataatta attcaactta ttcaattgat
      361 gatatgccag gattcctctt cctttatctt caatgtttcg attaacaggt ccattatccg
      421 gtttttagtt tcgctgcaca ttgccgaatg acaatcggat tcctttaagc tttgcttttg
      481 ccctgttttc tctccccagt aatcgttggg tgactgaacc atccagtagc cgagagttac
      541 gtttttcctg atttaataaa tatccatatc tatttgtctc gtggtgtttt acagcaacta
      601 accgaaatta ctattttgca tcatccaacc ataaagagac aacaataatt ctagaaaaca
      661 tataacaagt gtttttttct atttacgtct catacgcaaa gtccacttta ccatgttttg
      721 gaaacttgta cccaaccatt catttttcga ttttgtaatg aggctctttt cttctaacaa
      781 tcattgaggt cattatcatg tgtcctcacc tattgtttaa attgaggctt gcgctattcc
      841 caaaattgtg ggtattattt tcatatatca gtatctgcaa tttc
//
