LOCUS       sequence-151957-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow N-terminal tag(s) to 
            be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) 
            promoter, LEU2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP81xN_ccdB
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..271)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc agcttttttg tacaaacttg tcatatgact agtctcgagg tatgatttaa
      301 caaagcgact ataagtcaga aagtgagaat gagattgaaa taattaattc aacttattca
      361 attgatgata tgccaggatt cctcttccat cttcaatgtt tcgattaaca ggtccattat
      421 ccggttttta gtttcgctgc acattgccga atgacaatcg gattccttta agctttgctt
      481 ttgccctgtt ttctctcccc agtaatcgtt gggtgactga accatccagt agccgagagt
      541 tacgtttttc ctgatttaat aaatatccat atctatttgt ctcgtggtgt tttacagcaa
      601 ctaaccgaaa ttactatttt gcatcatcca accataaaga gacaacaata attctagaaa
      661 acatataaca agtgtttttt tctatttacg tctcatacgc aaagtccact ttaccatgtt
      721 ttggaaactt gtacccaacc attcattttt cgattttgta atgaggctct ttt
//
