LOCUS       sequence-151960-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow N-terminal tag(s) to 
            be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) 
            promoter, ura4+ selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP82xN_ccdB
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtcatat gactagtctc gaggtatgat ttaacaaagc
      301 gactataagt cagaaagtga gaatgagatt gaaataatta attcaactta ttcaattgat
      361 gatatgccag gattcctctt ccatcttcaa tgtttcgatt aacaggtcca ttatccggtt
      421 tttagtttcg ctgcacattg ccgaatgaca atcggattcc tttaagcttt gcttttgccc
      481 tgttttctct ccccagtaat cgttgggtga ctgaaccatc cagtagccga gagttacgtt
      541 tttcctgatt taataaatat ccatatctat ttgtctcgtg gtgttttaca gcaactaacc
      601 gaaattacta ttttgcatca tccaaccata aagagacaac aataattcta gaaaacatat
      661 aacaagtgtt tttttctatt tacgtctcat acgcaaagtc cactttacca tgttttggaa
      721 acttgtaccc aaccattcat ttttcgattt tgtaatgagg ctcttttctt ctaacaatca
      781 ttgaggtcat tatcatg
//
